标题：Sleeve Gastrectomy Attenuates Diabetic Nephropathy by Upregulating Nephrin Expressions in Diabetic Obese Rats
作者：Xiong, Yacheng; Zhu, Wei; Xu, Qian; Ruze, Rexiati; Yan, Zhibo; Li, Jianwen; Hu, Sanyuan; Zhong, Mingwei; Cheng, Yugang; Zhang, Guang 更多 作者机构：[Xiong, Yacheng; Xu, Qian; Ruze, Rexiati] Shandong Univ, Shandong Prov Qianfoshan Hosp, Dept Gen Surg, Jinan 250014, Shandong, Peoples R China.; [Zh 更多
通讯作者地址：Zhang, GY (corresponding author), Shandong First Med Univ, Dept Gen Surg, Affiliated Hosp 1, 16766 Jingshi Rd, Jinan 250014, Shandong, Peoples R China 更多
关键词：Sleeve gastrectomy; Diabetic nephropathy; Nephrin; Glomerular; ultrastructure
摘要：Purpose Diabetic nephropathy (DN) is the leading cause of end-stage renal disease, and sleeve gastrectomy (SG) is considered to be an effective strategy to improve pre-existing DN. However, the mechanism remains unknown. Materials and Methods Animal model of DN was induced by high-fat diet (HFD) and streptozotocin (STZ). SG or sham surgery was performed and rats were sacrificed at 4, 8, and 12 weeks after surgery. The basic parameters (blood glucose, body weight, kidney weight), indicators of renal function including serum creatinine (Scr), blood urea nitrogen (BUN), urine microalbumin, urine creatinine (Ucr), microalbumin creatinine ratio (UACR), ultrastructural changes of glomerulus, and the expression of nephrin gene and protein in glomerular podocytes were compared among groups. Results Blood glucose and body weight of SG rats were significantly lower than those of the sham-operated rats, and renal function of SG groups were also significantly improved within the postoperative period of 12 weeks. The results of periodic acid-Schiff staining (PAS) and transmission electron microscopy (TEM) showed that glomerular hypertrophy and accumulation of extracellular matrix proteins were significantly alleviated after SG, and the thickness of basement membrane and the fusion or effacement of foot processes were also significantly improved. The mRNA and protein expression of nephrin in SG groups was significantly higher than that in the sham group. Conclusion These results suggest that SG attenuates DN by upregulating the expression of nephrin and improving the ultrastructure of glomerular filtration membrane. This study indicates that SG can be used as an available therapeutic intervention for DN.