标题：Mutation directional selection sheds light on prion pathogenesis
作者：Shen, Liang; Ji, Hong-Fang
作者机构：[Shen, Liang; Ji, Hong-Fang] Shandong Univ Technol, Shandong Prov Res Ctr Bioinformat Engn & Tech, Zibo 255049, Peoples R China.
通讯作者地址：[Ji, HF]Shandong Univ Technol, Shandong Prov Res Ctr Bioinformat Engn & Tech, Zibo 255049, Peoples R China.
来源：BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
关键词：Prion pathogenesis; Mutation; Directional selection; Facilitating factor
摘要：As mutations in the PRNP gene account for human hereditary prion diseases (PrDs), it is crucial to elucidating how these mutations affect the central pathogenic conformational transition of normal cellular prion protein (PrP(C)) to abnormal scrapie isoform (PrP(Sc)). Many studies proposed that these pathogenic mutations may make PrP more susceptible to conformational change through altering its structure stability. By evaluating the most recent observations regarding pathogenic mutations, it was found that the pathogenic mutations do not exert a uniform effect on the thermodynamic stability of the human PrP's structure. Through analyzing the reported PrDs-related mutations, we found that 25 out of 27 mutations possess strong directional selection, i.e., enhancing hydrophobicity or decreasing negative and increasing positive charge. Based on the triggering role reported by previous studies of facilitating factors in PrP(C) conversion, e.g., lipid and polyanion, we proposed that the mutation-induced changes may strengthen the interaction between PrP and facilitating factors, which will accelerate PrP conversion and cause PrDs. (C) 2011 Elsevier Inc. All rights reserved.