标题:Ultrasensitive electrochemiluminescence method for determination of DNA using Ru(bpy)_3~2~+-coated magnetic submicrobeads wrapped with carbon nanotubes
作者:Liping Shen;Jun Li;Lu Li;Guizheng Zou;Xiaoli Zhang;Wenrui Jin
作者机构:[Shen, L] School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, China;[ Li, J] School of Chemistry and Chemical Engineering 更多
通讯作者:Jin, W
通讯作者地址:[Zou, GZ]Shandong Univ, Sch Chem & Chem Engn, Jinan 250100, Peoples R China.
来源:Electrochemistry communications
出版年:2011
卷:13
期:12
页码:1499-1501
DOI:10.1016/j.elecom.2011.10.002
关键词:Electrochemiluminescence assay;DNA detection;Microbead;Carbon nanotube
摘要:We developed an ultrasensitive electrochemiluminescence (ECL) method for DNA determination using magnetic submicrobeads (SMBs) as the carrier of Ru(bpy)_3 ~2~+ (bpy=2,2′-bipyridy) and carbon nanotubes (CNTs) as accessorial electrode material. The SMBs with Ru(bpy)_3 ~2~+ were wrapped with CNTs and then immobilized on an Au electrode. In the presence of tri-n-propylamine, ECL of the Ru(bpy)_3 ~2~+ on the SMBs was detected. Since one target DNA (t-DNA) molecule corresponded to one SMB with a large number of Ru(bpy)_3 ~2~+, the ECL signal was amplified. In addition, the Ru(bpy)_3 ~2~+-loaded SMBs were wrapped with CNTs that contacted the electrode. The ECL of Ru(bpy)_3 ~2~+ was greatly increased. Using this method, t-DNA of 3×10~(?16) mol/L could be detected. The method could be used to quantify mRNA in cells.
收录类别:EI;SCOPUS;SCIE
WOS核心被引频次:7
Scopus被引频次:7
资源类型:期刊论文
原文链接:https://www.scopus.com/inward/record.uri?eid=2-s2.0-84861197374&doi=10.1016%2fj.elecom.2011.10.002&partnerID=40&md5=e41950935cc6d7921c59af4ab284bbd5
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