标题：Ultrasensitive electrochemiluminescence method for determination of DNA using Ru(bpy)_3~2~+-coated magnetic submicrobeads wrapped with carbon nanotubes
作者：Liping Shen;Jun Li;Lu Li;Guizheng Zou;Xiaoli Zhang;Wenrui Jin
作者机构：[Shen, L] School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, China;[ Li, J] School of Chemistry and Chemical Engineering 更多
通讯作者地址：[Zou, GZ]Shandong Univ, Sch Chem & Chem Engn, Jinan 250100, Peoples R China.
关键词：Electrochemiluminescence assay;DNA detection;Microbead;Carbon nanotube
摘要：We developed an ultrasensitive electrochemiluminescence (ECL) method for DNA determination using magnetic submicrobeads (SMBs) as the carrier of Ru(bpy)_3 ~2~+ (bpy=2,2′-bipyridy) and carbon nanotubes (CNTs) as accessorial electrode material. The SMBs with Ru(bpy)_3 ~2~+ were wrapped with CNTs and then immobilized on an Au electrode. In the presence of tri-n-propylamine, ECL of the Ru(bpy)_3 ~2~+ on the SMBs was detected. Since one target DNA (t-DNA) molecule corresponded to one SMB with a large number of Ru(bpy)_3 ~2~+, the ECL signal was amplified. In addition, the Ru(bpy)_3 ~2~+-loaded SMBs were wrapped with CNTs that contacted the electrode. The ECL of Ru(bpy)_3 ~2~+ was greatly increased. Using this method, t-DNA of 3×10~(?16) mol/L could be detected. The method could be used to quantify mRNA in cells.