标题：Chaperone-mediated autophagy regulates apoptosis and the proliferation of colon carcinoma cells
作者：Peng, Jie-qiong; Han, Shu-mei; Chen, Ze-hao; Yang, Jing; Pei, Yan-qing; Bao, Cong; Qiao, Lei; Chen, Wen-qiang; Liu, Bo
作者机构：[Peng, Jie-qiong] Univ Jinan, Sch Med & Life Sci, Shandong Acad Med Sci, Jinan, Peoples R China.; [Peng, Jie-qiong; Han, Shu-mei; Yang, Jing; Pei, Y 更多
通讯作者：Chen, WQ;Chen, WQ;Liu, B
通讯作者地址：[Chen, WQ]Shandong Univ, Qilu Hosp, Key Lab Cardiovasc Remodeling & Funct Res, Chinese Minist Educ, 107 Wen Hua Xi Rd, Jinan 250012, Shandong, Peoples 更多
来源：BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
关键词：Colorectal cancer; Chaperone-mediated autophagy; Apoptosis;; Proliferation
摘要：Chaperone-mediated autophagy (CMA) is one of the three types of autophagy. In recent years, CMA has been shown to be associated with the pathogenesis of several types of cancer. However, whether CMA is involved in the pathogenesis of colorectal cancer (CRC) remains unclear. In this study, we investigated CMA activity in tissue specimens from CRC patients and mouse models of colitis-associated CRC (induced by administration of AOM plus DSS). In addition, we down-regulated CMA in CT26 colon carcinoma cells stably transfected with a vector expressing a siRNA targeting LAMP-2A, the limiting component in the CMA pathway, to explore the role of CMA in these cells. Apoptosis was detected using TUNEL assay, and the apoptosis-related proteins were detected using western blotting. Cell proliferation was assessed using MTT assay, Ki-67 labelling and western blotting for PCNA. We found that LAMP-2A expression was significantly increased in CRC patients and mouse models and varied according to the stage of the disease. Inhibition of CMA in CT26 cells facilitated apoptosis, as evidenced by increased TUNEL immunolabeling, increased expression of Bax and Bnip3, and decreased expression of Bcl-2. Cell proliferation assays showed that inhibition of CMA impeded the proliferation of CT26 cells. These data support the hypothesis that CMA is up-regulated in CRC, and inhibition of CMA may be a new therapeutic strategy for CRC patients. (C) 2019 Elsevier Inc. All rights reserved.