标题：Pyroptosis is involved in cryopreservation and auto-transplantation of mouse ovarian tissues and pyroptosis inhibition improves ovarian graft function
作者：Wang, Hong-Xia; Lu, Xi-Lan; Huang, Wen-Jie; Zhang, Jian-Min
作者机构：[Wang, Hong-Xia; Lu, Xi-Lan] Shandong Univ, Jinan Cent Hosp, Dept Reprod Med, Jinan, Shandong, Peoples R China.; [Huang, Wen-Jie] Shandong Xiehe Uni 更多
通讯作者地址：[Zhang, JM]Weifang Nursing Vocat Coll, Qingzhou City, Peoples R China.
来源：RESEARCH IN VETERINARY SCIENCE
关键词：Pyroptosis; Ovarian tissue; Cryopreservation; Transplantation
摘要：The aim of this study was to investigate the role of pyroptosis in the cryopreservation and transplantation of mouse ovarian tissues; the effects of pyroptosis inhibitior on the ovarian graft function were also explored. ICR (institute of cancer research) mice were randomly divided into control group and experimental groups (n = 10 per group). The experimental groups included fresh graft group (autograft), cryopreserved graft group (cryopreservation + autograft), and pyroptosis inhibition group (cryopreservation + autograft + pyroptosis inhibitor). At the third day after auto-transplantation, caspase-1 and NLRP3 protein expression in grafts were assessed by Western blot; in the meantime, serum concentration of IL-1 beta was examined by ELISA. After 28 days of auto-transplantation, estradiol concentrations and follicular densities of grafts were evaluated. The caspase-1 and NLRP3 protein expression in grafts from all the experimental groups were significantly higher than that from control group respectively; moreover, there was a significant increase in serum concentrations of IL-1 beta in all experimental groups compared with control group. The concentration of estradiol and follicular densities of grafts in pyroptosis inhibition group were significantly higher than that in cryopreserved graft group. Pyroptosis is involved in cryopreservation and auto-transplantation of mouse ovarian tissues, and pyroptosis inhibition can improve the ovarian graft function.