标题:Absence or mislocalization of DNAH5 is a characteristic marker for motile ciliary abnormality in nasal polyps
作者:Qiu, Qianhui; Peng, Yang; Zhu, Zhenchao; Chen, Zhuo; Zhang, Chi; Ong, Hsiao Hui; Sen Tan, Kai; Hong, Haiyu; Yan, Yan; Huang, Haoqi; 更多
通讯作者:Wang, DY
作者机构:[Qiu, Qianhui; Peng, Yang] Southern Med Univ, Zhujiang Hosp, Dept Otolaryngol, Guangzhou, Guangdong, Peoples R China.; [Huang, Haoqi] Southern Med U 更多
会议名称:Rhinology World Congress
会议日期:SEP 01-03, 2017
来源:LARYNGOSCOPE
出版年:2018
卷:128
期:3
页码:E97-E104
DOI:10.1002/lary.26983
关键词:Cilia; dynein axonemal heavy chain 5 (DNAH5); expression pattern;; inflammation; nasal polyps (NPs)
摘要:ObjectiveMotile cilia impairment is a common condition in patients with chronically inflamed airways, such as is seen in nasal polyps (NPs). The mechanism underlying this pathogenic condition is complex and not fully understood.; MethodsWe investigated the presence and localization of dynein axonemal heavy chain 5 (DNAH5) in motile cilia using immunofluorescence staining in paraffin-embedded nasal biopsies from NPs (n = 120) and inferior turbinate mucosa (n = 35) of healthy controls. We also performed single-cell staining on cytospin samples (NP = 5, control = 5). Three patterns of DNAH5 localization are defined, including pattern A (presence throughout the axoneme), pattern B (undetectable in the distal part of the axoneme), and pattern C (completely missing throughout the entire axoneme). We developed a semiquantitative scoring system for which 0 = (pattern A > 70%); 1 = (patterns A + B > 70%); and 2 = (pattern C 30%) in each high-power field (5 fields per sample).; ResultsBased on our DNAH5 scoring system, the median (1st and 3rd quartile) score was 0.3 (0.2 and 0.4) for samples from controls, and 1.1 (0.6 and 1.6) for samples from NPs in paraffin specimens (P < 0.001). The DNAH5 score had a significant positive relationship with the Lund-Mackay computed tomography score (r = 0.329, P = 0.005) and was higher in patients with eosinophilic NPs (P = 0.006). For cytospin samples, the mean percentage of patterns A, B, and C were 74%, 14%, and 12% in controls, and 48%, 20%, and 32% in NPs, respectively.; ConclusionOur results suggest that the absence or mislocalization of DNAH5 from motile cilia is a common and potentially important pathological phenomenon in chronically inflamed airway epithelium.; Level of EvidenceNA. Laryngoscope, 128:E97-E104, 2018
收录类别:CPCI-S;SCOPUS;SCIE
WOS核心被引频次:1
Scopus被引频次:1
资源类型:会议论文;期刊论文
原文链接:https://www.scopus.com/inward/record.uri?eid=2-s2.0-85034221066&doi=10.1002%2flary.26983&partnerID=40&md5=13b8cf99f7943dbfb4ed04eeabadcfc1
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