标题：Electrochemiluminescence immunoassay at a nanoporous gold leaf electrode and using CdTe quantun dots as labels
作者：Li, Xiaoyu; Wang, Rongyue; Zhang, Xiaoli
作者机构：[Li, Xiaoyu; Wang, Rongyue; Zhang, Xiaoli] Shandong Univ, Sch Chem & Chem Engn, Jinan 250100, Peoples R China.
通讯作者地址：[Zhang, XL]Shandong Univ, Sch Chem & Chem Engn, Jinan 250100, Peoples R China.
关键词：Electrochemiluminescence; Immunoassay; Quantum dots; Nanoporous gold; leaf electrode; Carcinoembryonic antigen
摘要：An electrochemiluminescence-based immunoassay using quantum dots (QDs) as labels for the carcinoembryonic antigen (CEA) was developed using an electrode modified with leafs of nanoporous gold. CEA was initially immobilized on the electrode via a sandwich immunoreaction, and then CdTe quantum dots capped with thioglycolic acid were used to label the second antibody. The intensity of the ECL of the QDs reflects the quantity of CEA immobilized on the electrode. Thus, in the presence of dithiopersulfate as the coreactant, the ECL serves as the signal for the determination of CEA. The intensity of the electroluminescence (ECL) of the electrode was about 5.5-fold higher than that obtained with a bare gold electrode. The relation between ECL intensity and CEA concentration is linear in the range from 0.05 to 200 ng.mL(-1), and the detection limit is 0.01 ng.mL(-1). The method has the advantages of high sensitivity, good reproducibility and long-term stability, and paves a new avenue for applying quantum dots in ECL-based bioassays.