标题:A sandwich-type electrochemical immunosensor based on RhPt NDs/NH2-GS and Au NPs/PPy NS for quantitative detection hepatitis B surface antigen
作者:Pei, Fubin ;Wang, Ping ;Ma, Enhui ;Yang, Qingshan ;Yu, Haoxuan ;Gao, Chunxiao ;Li, Yueyun ;Liu, Qing ;Dong, Yunhui
作者机构:[Pei, Fubin ;Wang, Ping ;Ma, Enhui ;Yang, Qingshan ;Yu, Haoxuan ;Gao, Chunxiao ;Li, Yueyun ;Liu, Qing ;Dong, Yunhui ] School of Chemistry and Chemical 更多
通讯作者:Wang, Ping
来源:Bioelectrochemistry
出版年:2019
卷:126
页码:92-98
DOI:10.1016/j.bioelechem.2018.11.008
摘要:In this work, a sandwich-type electrochemical immunosensor was fabricated to quantitatively detect hepatitis B surface antigen (HBsAg). The immunosensor was based on Rh core and Pt shell nanodendrites loaded onto amino group functionalized graphene nanosheet (RhPt NDs/NH2-GS) as label and gold nanoparticles loaded onto polypyrrole nanosheet (Au NPs/PPy NS) as platform. RhPt NDs with abundant catalytic active sites because of the branched core-shell structure, RhPt NDs/NH2-GS as the label displayed high catalytic activity, amplifying the current signal of the immunosensor. Additionally, Au NPs/PPy NS enhanced the electron transfer and provided a good microenvironment to immobilize antibodies effectively, thus improving the sensitivity of the immunosensor. Based on above advantages, the immunosensor emerged a linear concentration ranging from 0.0005 to 10 ng/mL, a low detection limit of 166 fg/mL for HBsAg (S/N = 3) and good stability, selectivity, reproducibility. Furthermore, the satisfactory accuracy in analysis of actual serum samples implied the immunosensor had promising prospect in clinical analysis applications.
© 2018 Elsevier B.V.
收录类别:EI
资源类型:期刊论文
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