标题：Development of wide-angle 2D light scattering static cytometry
作者：Xie, Linyan; Liu, Qiao; Shao, Changshun; Su, Xuantao
作者机构：[Xie, Linyan; Su, Xuantao] Shandong Univ, Sch Control Sci & Engn, Inst Biomed Engn, Jinan 250061, Peoples R China.; [Liu, Qiao; Shao, Changshun] Sha 更多
会议名称：Conference on Optics in Health Care and Biomedical Optics VII
会议日期：OCT 12-14, 2016
来源：OPTICS IN HEALTH CARE AND BIOMEDICAL OPTICS VII
关键词：Static cytometer; wide-angle; 2D light scattering; label-free; single; cell
摘要：We have recently developed a 2D light scattering static cytometer for cellular analysis in a label-free manner, which measures side scatter (SSC) light in the polar angular range from 79 to 101 degrees. Compared with conventional flow cytometry, our cytometric technique requires no fluorescent labeling of the cells, and static cytometry measurements can be performed without flow control. In this paper we present an improved label-free static cytometer that can obtain 2D light scattering patterns in a wider angular range. By illuminating the static microspheres on chip with a scanning optical fiber, wide-angle 2D light scattering patterns of single standard microspheres with a mean diameter of 3.87 mu m are obtained. The 2D patterns of 3.87 mu m microspheres contain both large-angle forward scatter (FSC) and SSC light in the polar angular range from 40 to 100 degrees, approximately. Experimental 2D patterns of 3.87 mu m microspheres are in good agreement with Mie theory simulated ones. The wide-angle light scattering measurements may provide a better resolution for particle analysis as compared with the SSC measurements. Two dimensional light scattering patterns of HL-60 human acute leukemia cells are obtained by using our static cytometer. Compared with SSC 2D light scattering patterns, wide-angle 2D patterns contain richer information of the HL-60 cells. The obtaining of 2D light scattering patterns in a wide angular range could help to enhance the capabilities of our label-free static cytometry for cell analysis.