标题:DETERMINATION OF PROTOBERBERINE ALKALOIDS IN COPTIS CHINENSIS BY MICROEXTRACTION AND HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
作者:Liu, Xi; Xing, Rongrong; Chen, Xuan; Hu, Shuang; Sheng, Xia; Bai, Xiaohong
作者机构:[Liu, Xi; Xing, Rongrong; Chen, Xuan; Hu, Shuang; Bai, Xiaohong] Shanxi Med Univ, Coll Pharm, Taiyuan 030001, Peoples R China.; [Sheng, Xia] Shandon 更多
通讯作者:Bai, XH
通讯作者地址:[Bai, XH]Shanxi Med Univ, Coll Pharm, Taiyuan 030001, Peoples R China.
来源:ANALYTICAL LETTERS
出版年:2014
卷:47
期:16
页码:2655-2664
DOI:10.1080/00032719.2014.921820
关键词:Coptis chinensis; Filter membrane; Microextraction; Protoberberine; alkaloids
摘要:Sample preparation technique based on an organic filter membrane (pH-resolved filter membrane microextraction) (pH-RFMME) was developed, coupled with high-performance liquid chromatography, and used to determine protoberberine alkaloids (jatrorrhizine, epiberberine, coptisine, palmatine, and berberine) in Coptis chinensis at different pH values through a one-step procedure. This green procedure provides a desirable sample pretreatment technology. The main variables affecting the extraction such as filter membrane area (or volumes of extraction solvents), sample pH, eluent pH, ionic strength, extraction stirring rate, extraction time, and sample volume were optimized. Under the optimized conditions, the enrichment factors of the analytes were 40.4-52.0, the linear ranges were 3.2-6250 ng.mL(-1) for jatrorrhizine and epiberberine, 6.0-12000 ng.mL(-1) for coptisine, 1.8-3600 ng.mL(-1) for palmatine, and 18.8-18800 ng.mL(-1) for berberine, with r(2) >= 0.9945. The limits of detection were less than 0.3 ng.mL(-1). Satisfactory recoveries (84.8%-115.5%) and precision (1.8%-10.0%) were also achieved. These results confirmed that pH-RFMME is a simple, rapid, practical, and environmentally friendly method to isolate analytes that exhibit significant differences in acidity or alkalinity from complex samples.
收录类别:SCOPUS;SCIE
WOS核心被引频次:3
Scopus被引频次:3
资源类型:期刊论文
原文链接:https://www.scopus.com/inward/record.uri?eid=2-s2.0-84907581745&doi=10.1080%2f00032719.2014.921820&partnerID=40&md5=6ef6f0cc877edece5bf1d35126b566f1
TOP