标题：Size-Dependent Cell Uptake of Protein-Coated Graphene Oxide Nanosheets
作者：Qingxin Mu;Gaoxing Su;Liwen Li
作者机构：[Mu, Q] Department of Chemical Biology and Therapeutics, St. Jude Children's Research Hospital, Memphis, TN 38105, United States;[ Su, G] Department o 更多
通讯作者地址：[Yan, B]St Jude Childrens Res Hosp, Dept Chem Biol & Therapeut, 332 N Lauderdale, Memphis, TN 38105 USA.
来源：ACS applied materials & interfaces
关键词：graphene oxide nanosheets;protein binding;cell uptake;clathrin-mediated endocytosis;phagocytosis;size dependence
摘要：As an emerging applied material, graphene has shown tremendous application potential in many fields, including bio-medicine. However, the biological behavior of these nanosheets, especially their interactions with cells, is not well understood. Here, we report our findings about the cell surface adhesion, subcellular locations, and size-dependent uptake mechanisms of protein-coated graphene oxide nanosheets (PCGO). Small nanosheets enter cells mainly through clathrin-mediated endocytosis, and the increase of graphene size enhances phagocytotic uptake of the nanosheets. These findings will facilitate biomedical and toxicologic studies of graphenes and provide fundamental understanding of interactions at the interface of two-dimensional nanostructures and biological systems.