标题:Heterologous expression of Shigella dysenteriae serotype 1 O-antigen analog in Escherichia coli K-12 W3110 by transferring a minimum number of genes involved in O-polysaccharide biosynthesis
作者:Kong, Yun; Qu, Yajun; Wang, Shengjun; Wang, Peng George; Chen, Min
作者机构:[Kong, Yun; Qu, Yajun; Wang, Shengjun; Wang, Peng George; Chen, Min] Shandong Univ, Natl Glycoengn Res Ctr, State Key Lab Microbial Technol, Jinan 250 更多
通讯作者:Chen, Min
通讯作者地址:[Chen, M]Shandong Univ, Natl Glycoengn Res Ctr, State Key Lab Microbial Technol, Jinan 250100, Shandong, Peoples R China;[Chen, M]Shandong Univ, Sch L 更多
来源:BIOTECHNOLOGY LETTERS
出版年:2018
卷:40
期:8
页码:1219-1226
DOI:10.1007/s10529-018-2584-6
关键词:E. coli K-12 W3110; Glycosyltransferase; Heterologous O-antigen; expression; O-antigen; O-polysaccharide; Shigella dysenteriae 1
摘要:To heterologously produce the Shigella dysenteriae serotype 1 O-polysaccharide (O-PS, O-antigen) in Escherichia coli by transferring the minimum number of genes instead of the entire O-PS gene cluster.; The three glycosyltransferase genes (rfbR, rfbQ and rfp) responsible for the formation of the O-repeat unit were introduced into E. coli K-12 W3110 to synthesize S. dysenteriae 1 O-PS. The specific O-antigen ladder type with different chain lengths of O-repeat units was observed in the recombinant E. coli strain by SDS-PAGE silver staining and western blotting using S. dysenteriae 1 lipopolysaccharide antiserum. Analysis by mass spectrometry and ion chromatography suggested generation of the specific S. dysenteriae 1 O-repeat unit structure with an extra glucose residue attached.; Recombinant E. coli expressing specific glycosyltransferase genes can generate the O-PS of S. dysenteriae 1 and might be able to synthesize heterologous O-antigens of various pathogenic bacteria for vaccine preparation.
收录类别:EI;SCOPUS;SCIE
资源类型:期刊论文
原文链接:https://www.scopus.com/inward/record.uri?eid=2-s2.0-85048798695&doi=10.1007%2fs10529-018-2584-6&partnerID=40&md5=6f7aec37eab1d7607d0c2a44501eaec7
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