标题:Calixarene and ionic liquid assisted matrix solid-phase dispersion microextraction of organic acids from fruit
作者:Yang, Juan; Dong, Xin; Hu, Yu-Han; Wang, Qiu-Yan; Wang, Shu-Ling; Cao, Jun; Zhang, Hong-Hua
通讯作者:Cao, J;Wang, SL;Zhang, HH;Wang, ShuLing
作者机构:[Yang, Juan; Dong, Xin; Hu, Yu-Han; Wang, Qiu-Yan; Cao, Jun] Hangzhou Normal Univ, Coll Mat Chem & Chem Engn, Hangzhou 311121, Zhejiang, Peoples R Chi 更多
会议名称:47th International Symposium on High Performance Liquid Phase Seperations and Related Techniques (HPLC)
会议日期:JUL 29-AUG 02, 2018
来源:JOURNAL OF CHROMATOGRAPHY A
出版年:2019
卷:1602
页码:150-159
DOI:10.1016/j.chroma.2019.03.055
关键词:Calixarene; Chaenomeles speciosa; Ionic liquid; Matrix solid-phase; dispersion; Organic acids; UH PLC-Q-TOF/MS
摘要:A rapid and effective method was successfully established for the extraction and determination of chlorogenic acid, protocatechuic acid, malic acid, caffeic acid and gallic acid in fruit (chaenomeles speciosa) via matrix solid-phase dispersion (MSPD) microextraction combined with ultra-high performance liquid chromatography with quadrupole time-of-flight tandem mass spectrometry (UHPLC-Q-TOF/MS). Several major extraction parameters were investigated and optimized, such as the type of sorbent, the amount of sorbent, the grinding time, the type and concentration of the eluting solvent. The optimal extraction conditions were obtained by using 20 mg of calix[8]arene as dispersing adsorbent, selecting 60s as the appropriate grinding time and applying 250 mM of 1-dodecyl-3-methylimidazolium bromide as eluent solvent. Moreover, the calibration curves of the analytes were in the range of 0.01-500 mu g/mL with the determination coefficients (r(2)) higher than 0.9995. The limits of detection and limits of quantification were in the range of 0.202-1.056 ng/mL and 0.674-3.521 ng/mL, respectively. The recoveries of the target compounds at two spiked levels were between 82.19 and 113.36%. Furthermore, this method had acceptable reproducibility (RSD <= 3.84%). The proposed approach combined the advantages of MSPD microextraction with UHPLC-Q-TOF/MS, and could be applicable for the analysis organic acids in fruit. (C) 2019 Elsevier B.V. All rights reserved.
收录类别:CPCI-S;EI;SCIE
资源类型:会议论文;期刊论文
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