标题：Polyhydroxyalkanoic acids from structurally-unrelated carbon sources in Escherichia coli
作者：Wang, Qian; Zhuang, Qianqian; Liang, Quanfeng; Qi, Qingsheng
作者机构：[Wang, Qian; Zhuang, Qianqian; Liang, Quanfeng; Qi, Qingsheng] Shandong Univ, State Key Lab Microbial Technol, Natl Glycoengn Res Ctr, Jinan 250100, P 更多
通讯作者地址：[Qi, QS]Shandong Univ, State Key Lab Microbial Technol, Natl Glycoengn Res Ctr, Jinan 250100, Peoples R China.
来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
关键词：Polyhydroxyalkanoates; Unrelated carbon source; Escherichia coli; PHA;; Metabolic engineering
摘要：Polyhydroxyalkanoates (PHAs) that contain varied monomers with different chain lengths/structures were normally synthesized when a structurally-related precursor was present. The biosynthesis of PHAs from unrelated carbon sources in microorganisms including Escherichia coli met many challenges in the past. Recently, with the development of metabolic engineering and synthetic biology, the production of PHAs from unrelated carbon sources obtained a breakthrough. Polyesters containing 2-hydroxypropionate, 3-hydroxypropionate, 4-hydroxybutyrate, 3-hydroxyvalarate, and medium-chain-length 3-hydroxyalkanoate monomers can all be synthesized in E. coli by integrating exogenous or endogenous pathways and/or genes. This review will summarize the progresses in this area. In addition, the strategies that lead to the production of PHAs with varied monomers and high polymer content in the cell are discussed.