标题：Ultrasensitive Immunoassay Based on Anodic Near-Infrared Electrochemiluminescence from Dual-Stabilizer-Capped CdTe Nanocrystals
作者：Guodong Liang;Shufeng Liu;Guizheng Zou;Xiaoli Zhang
作者机构：[Liang, G] School of Chemistry and Chemical Engineering, Shandong University, Jinan, 250100, China;[ Liu, S] School of Chemistry and Chemical Engineer 更多
通讯作者地址：[Zou, GZ]Shandong Univ, Sch Chem & Chem Engn, Jinan 250100, Peoples R China.
摘要：A sandwich-typed near-infrared (NIR) electrochemiluminescence (ECL) immunoassay was developed with dual-stabilizer-capped CdTe nanocrystals (NCs) as ECL labels and a fetoprotein antigen (AFP) as model protein. The dual-stabilizer-capped NIR CdTe NCs were promising ECL labels because of their NIR ECL emission of 800 nm, low anodic ECL potential of +0.85 V, and high biocompatibity, which can facilitate interference-free and highly sensitive ECL bioassays. Upon the immunorecognition of the immobilized AFP to its antibody labeled with dual-stabilizer-capped CdTe NCs, the proposed immunoassay displayed increasing ECL intensity, leading to a wide calibration range of 10.0 pg/mL to 80.0 ng/mL with a detection limit of 5.0 pg/mL [signal-to-noise ratio (S/N) = 3] without coupling any signal amplification procedures. The NIR ECL immunoassay for real samples displayed very similar results with those of Ru(bpy)_3~(2+) reagent kit based commercial ECL immunoassay, which not only proved for the efficiency of NIR ECL from dual-stabilizer-capped CdTe NCs but also paved the road for development of novel ECL emitters and corresponding reagent kits.