标题:Ultrasensitive Immunoassay Based on Anodic Near-Infrared Electrochemiluminescence from Dual-Stabilizer-Capped CdTe Nanocrystals
作者:Guodong Liang;Shufeng Liu;Guizheng Zou;Xiaoli Zhang
作者机构:[Liang, G] School of Chemistry and Chemical Engineering, Shandong University, Jinan, 250100, China;[ Liu, S] School of Chemistry and Chemical Engineer 更多
通讯作者:Zou, G
通讯作者地址:[Zou, GZ]Shandong Univ, Sch Chem & Chem Engn, Jinan 250100, Peoples R China.
来源:Analytical chemistry
出版年:2012
卷:84
期:24
页码:10645-10649
DOI:10.1021/ac302236a
摘要:A sandwich-typed near-infrared (NIR) electrochemiluminescence (ECL) immunoassay was developed with dual-stabilizer-capped CdTe nanocrystals (NCs) as ECL labels and a fetoprotein antigen (AFP) as model protein. The dual-stabilizer-capped NIR CdTe NCs were promising ECL labels because of their NIR ECL emission of 800 nm, low anodic ECL potential of +0.85 V, and high biocompatibity, which can facilitate interference-free and highly sensitive ECL bioassays. Upon the immunorecognition of the immobilized AFP to its antibody labeled with dual-stabilizer-capped CdTe NCs, the proposed immunoassay displayed increasing ECL intensity, leading to a wide calibration range of 10.0 pg/mL to 80.0 ng/mL with a detection limit of 5.0 pg/mL [signal-to-noise ratio (S/N) = 3] without coupling any signal amplification procedures. The NIR ECL immunoassay for real samples displayed very similar results with those of Ru(bpy)_3~(2+) reagent kit based commercial ECL immunoassay, which not only proved for the efficiency of NIR ECL from dual-stabilizer-capped CdTe NCs but also paved the road for development of novel ECL emitters and corresponding reagent kits.
收录类别:EI;SCOPUS;SCIE
WOS核心被引频次:55
Scopus被引频次:53
资源类型:期刊论文
原文链接:https://www.scopus.com/inward/record.uri?eid=2-s2.0-84871274530&doi=10.1021%2fac302236a&partnerID=40&md5=9000f27573f61cb962a913a881d9b28e
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