标题:TLR-induced NF-κB activation regulates NLRP3 expression in murine macrophages
作者:Qiao,Y.;Wang,P.;Qi,J.;Zhang,L.;Gao,C.
作者机构:[Qiao, Y] Key Laboratory for Experimental Teratology, Shandong University Medical School, Ministry of Education, Jinan, Shandong 250012, China, Depart 更多
通讯作者:Gao, CJ
通讯作者地址:[Gao, CJ]Shandong Univ, Sch Med, Dept Immunol, 44 Wenhua Xi Rd, Jinan 250012, Shandong, Peoples R China.
来源:FEBS Letters: For the Rapid Publication of Short Reports in Biochemistry, Biophysics and Molecular Biology
出版年:2012
卷:586
期:7
页码:1022-1026
DOI:10.1016/j.febslet.2012.02.045
关键词:Macrophage;NF-κB;NLRP3;Toll-like receptor
摘要:NLRP3 inflammasome plays a critical role in the activation of caspase-1 and maturation of IL-1β. However, the specific cis- and trans-regulatory elements that determine the extent of NLRP3 expression are not well defined. In this study, we found NLRP3 expression was induced by TLR agonists in murine macrophages in a NF-κB dependent manner. Furthermore, the corresponding NF-κB binding sites (nt -1303 to -1292 and -1238 to -1228) were identified in the NLRP3 promoter. Finally, EMSA and ChIP assays demonstrated LPS-induced NF-κB binding to the NLRP3 promoter. Therefore, out results delineated the molecular mechanisms involved in TLR-induced transcriptional regulation of NLRP3.
收录类别:SCOPUS;SCIE
WOS核心被引频次:83
Scopus被引频次:85
资源类型:期刊论文
原文链接:https://www.scopus.com/inward/record.uri?eid=2-s2.0-84862807232&doi=10.1016%2fj.febslet.2012.02.045&partnerID=40&md5=a2df183e47f75105fb84fde6659e4fc3
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