标题：TLR-induced NF-κB activation regulates NLRP3 expression in murine macrophages
作者机构：[Qiao, Y] Key Laboratory for Experimental Teratology, Shandong University Medical School, Ministry of Education, Jinan, Shandong 250012, China, Depart 更多
通讯作者地址：[Gao, CJ]Shandong Univ, Sch Med, Dept Immunol, 44 Wenhua Xi Rd, Jinan 250012, Shandong, Peoples R China.
来源：FEBS Letters: For the Rapid Publication of Short Reports in Biochemistry, Biophysics and Molecular Biology
摘要：NLRP3 inflammasome plays a critical role in the activation of caspase-1 and maturation of IL-1β. However, the specific cis- and trans-regulatory elements that determine the extent of NLRP3 expression are not well defined. In this study, we found NLRP3 expression was induced by TLR agonists in murine macrophages in a NF-κB dependent manner. Furthermore, the corresponding NF-κB binding sites (nt -1303 to -1292 and -1238 to -1228) were identified in the NLRP3 promoter. Finally, EMSA and ChIP assays demonstrated LPS-induced NF-κB binding to the NLRP3 promoter. Therefore, out results delineated the molecular mechanisms involved in TLR-induced transcriptional regulation of NLRP3.