标题：A C-type lectin with an immunoglobulin-like domain promotes phagocytosis of hemocytes in crayfish Procambarus clarkii
作者：Zhang, Xiao-Wen; Wang, Yue; Wang, Xian-Wei; Wang, Lei; Mu, Yi; Wang, Jin-Xing
作者机构：[Zhang, Xiao-Wen; Wang, Yue] Henan Normal Univ, Coll Life Sci, Xinxiang 453007, Henan, Peoples R China.; [Wang, Xian-Wei; Wang, Lei; Mu, Yi; Wang, J 更多
通讯作者：Wang, XW;Wang, JX
通讯作者地址：[Wang, XW; Wang, JX]Shandong Univ, Sch Life Sci, Shandong Prov Key Lab Anim Cells & Dev Biol, Jinan 250100, Shandong, Peoples R China.
摘要：C-type lectins are important immune molecules that participate in host defense response. The present work reports a novel C-type lectin (PcLec3) from the red swamp crayfish Procambarus clarkii. Sequence analysis found that PcLec3 encodes a polypeptide with252 amino acid residues, which contains an immunoglobulin-like domain (IG) and a C-type lectin domain (CTLD) arranged in tandem. Tissue distribution analysis indicated that PcLec3 is enriched expressed in hemocytes and hepatopancreas cells, in which PcLec3 was up-regulated following bacterial challenge by Vibrio anguillarum. Function analysis using recombinant full-length PcLec3, IG, and CTLD proteins revealed that these recombinant proteins had the capacity to bind carbohydrates and bacteria, while IG determined the cell binding activity. However, only full-length PcLec3 promotes the phagocytic activity of hemocytes and subsequent clearance of invasive bacteria. Taken together, these results manifest that PcLec3 acts as a hemocyte adhesion molecule to promote hemocyte phagocytosis against invasive V. anguillarum.