标题:Expression of microRNA-99a-3p in Prostate Cancer Based on Bioinformatics Data and Meta-Analysis of a Literature Review of 965 Cases
作者:Yan, Hai-biao; Zhang, Yu; Cen, Jie-mei; Wang, Xiao; Gan, Bin-liang; Huang, Jia-cheng; Li, Jia-yi; Song, Qian-hui; Li, Sheng-hua; Che 更多
作者机构:[Yan, Hai-biao; Li, Sheng-hua] Guangxi Med Univ, Affiliated Hosp 1, Dept Urol, Nanning, Guangxi, Peoples R China.; [Zhang, Yu; Cen, Jie-mei; Gan, Bi 更多
通讯作者:Li, SH
通讯作者地址:[Li, SH]Guangxi Med Univ, Affiliated Hosp 1, Dept Urol, Nanning, Guangxi, Peoples R China.
来源:MEDICAL SCIENCE MONITOR
出版年:2018
卷:24
页码:4807-4822
DOI:10.12659/MSM.908057
关键词:Afferent Pathways; MicroRNAs; Prostatic Neoplasms
摘要:Background: microRNAs (miRNAs) have a role as biomarkers in human cancer. The aim of this study was to use bioinformatics data, and review of cases identified from the literature, to investigate the role of microRNA-99a-3p (miR-99a-3p) in prostate cancer, including the identification of its target genes and signaling pathways.; Material/Methods: Meta-analysis from a literature review included 965 cases of prostate cancer. Bioinformatics databases interrogated for miR-99a-3p in prostate cancer included The Cancer Genome Atlas (TCGA), the Gene Expression Omnibus (GEO), and ArrayExpress. Twelve computational predictive algorithms were developed to integrate miR-99a-3p target gene prediction data. Bioinformatics analysis data from Gene Ontology (GO), the Kyoto Encyclopedia of Genes and Genomes (KEGG), and protein-protein interaction (PPI) network analysis were used investigate the possible pathways and target genes for miR-99a-3p in prostate cancer.; Results: TCGA data showed that miR-99a was down-regulated in prostate cancer when compared with normal prostate tissue. Receiver-operating characteristic (ROC) curve area under the curve (AUC) for miR-99a-3p was 0.660 (95% CI, 0.587-0.732) or a moderate level of discriminations. Pathway analysis showed that miR-99a-3p was associated with the Wnt and vascular endothelial growth factor (VEGF) signaling pathways. The PPP3CA and HYOU1 genes, selected from the PPI network, were highly expressed in prostate cancer tissue compared with normal prostate tissue, and negatively correlated with the expression of miR-99a-3p.; Conclusions: In prostate cancer, miR-99a-3p expression was associated with the Wnt and VEGF signaling pathways, which might inhibit the expression of PPP3CA or HYOU1.
收录类别:SCOPUS;SCIE
资源类型:期刊论文
原文链接:https://www.scopus.com/inward/record.uri?eid=2-s2.0-85050159222&doi=10.12659%2fMSM.908057&partnerID=40&md5=ef2ec4ddbcee3a400da0e14ff59ec618
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