标题:Equine immunoglobulin F(ab′)2 fragments protect mice from Rift Valley fever virus infection
作者:Zhao Y.; Zheng X.; He S.; Li Y.; Wang W.; Gai W.; Wong G.; Wang H.;等 更多
作者机构:[Zhao, Y] Institute of Military Veterinary Medicine, Academy of Military Medical Science, Changchun, 130122, China, Jiangsu Co-innovation Center for P 更多
通讯作者:Yang, S(yst62041@163.com)
通讯作者地址:[Yang, S] Department of Virology, Institute of Military Veterinary Medicine, Academy of Military Medical Science, 666 Liuying West Road, China;
来源:International Immunopharmacology
出版年:2018
卷:64
页码:217-222
DOI:10.1016/j.intimp.2018.09.002
关键词:Equine immunoglobulin F(ab′)2 fragments; Rift Valley fever virus; Vaccine preparation; Virus-like particle
摘要:Background: Rift Valley fever virus (RVFV) is an emerging arbovirus in Africa and the Arabian Peninsula, in which infection with RVFV poses a serious threat to humans and livestock globally. Approved treatments for RVFV infection, especially for use in humans, have not yet been developed. There is an urgent need for effective drugs to prevent RVFV disease. Methods: In previous study, we developed RVFV virus like particles (VLPs) expressing the surface glycoproteins Gn and Gc. The morphology was shown to be similar to live RVFV under electron microscopy. In this study, we immunized horses with RVFV VLPs, prepared the immunoglobulin F(ab′)2 fragments, and characterized its in vitro neutralization and in vivo efficacy in mice. Results: F(ab′)2 was found to potently neutralize RVFV in VeroE6 cells, and passive transfer of immunoglobulin F(ab′)2 fragments resulting in reduced mortality in RVFV infected mice. Conclusion: Our results show that passive immunotherapy with equine immunoglobulin F(ab′)2 fragments is a promising strategy to treat RVFV infections. © 2018
收录类别:SCOPUS
资源类型:期刊论文
原文链接:https://www.scopus.com/inward/record.uri?eid=2-s2.0-85052998121&doi=10.1016%2fj.intimp.2018.09.002&partnerID=40&md5=dfd5bd022d70ad01752b11ac0ef1df8d
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