标题:Cyclic voltammetry: A new strategy for the evaluation of oxidative damage to bovine insulin
作者:Zong, Wansong; Liu, Rutao; Sun, Feng; Wang, Meijie; Zhang, Pengjun; Liu, Yihong; Tian, Yanmin
作者机构:[Zong, Wansong; Liu, Rutao; Sun, Feng; Wang, Meijie; Zhang, Pengjun; Liu, Yihong; Tian, Yanmin] Shandong Univ, Sch Environm Sci & Engn, Jinan 250100, 更多
通讯作者:Liu, RT
通讯作者地址:[Liu, RT]Shandong Univ, Sch Environm Sci & Engn, Jinan 250100, Peoples R China.
来源:PROTEIN SCIENCE
出版年:2010
卷:19
期:2
页码:263-268
DOI:10.1002/pro.313
关键词:bovine insulin; oxidative damage; evaluation strategy; cyclic; voltammetry
摘要:Research on protein oxidative damage may give insight into the nature of protein functions and pathological conditions. In this work, the oxidative damage of bovine insulin on Au electrode was investigated by cyclic voltammetry (CV). The experimental results show that there are two anodic peaks for the oxidative damage of bovine insulin, which arise from the oxidation of the exposed disulfide bond SAS(CYS7A,CYS7B), forming sulfenic acid RSOH (1.20 V, vs. SCE), sulfinic acid RSO(2)H and sulfonic acid RSO(3)H (1.35 V, vs. SCE). These in vitro findings not only demonstrate the applicability of CV in simulating/evaluating the oxidative damage of nonredox proteins but also find two promising candidates (two anodic peaks) for measuring insulin.
收录类别:SCOPUS;SCIE
WOS核心被引频次:12
Scopus被引频次:13
资源类型:期刊论文
原文链接:https://www.scopus.com/inward/record.uri?eid=2-s2.0-76749121332&doi=10.1002%2fpro.313&partnerID=40&md5=7bf13bf53a24560c4d96605c4c31f748
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