标题：NCPP treatment alleviates ConA-induced hepatitis via reducing CD4+T activation and NO production
作者：Liu, Chenghu; Zhang, Lining; Gao, Shangxian; Qu, Zhonghua; Wang, Qun; Zhu, Faliang; Guo, Chun; Hou, Li; Wu, Ping; Shi, Yanping
作者机构：[Liu, Chenghu; Zhang, Lining; Wang, Qun; Zhu, Faliang; Guo, Chun; Hou, Li; Wu, Ping; Shi, Yanping] Shandong Univ, Sch Med, Dept Immunol, Shandong Qual 更多
通讯作者地址：[Zhang, LN]Shandong Univ, Sch Med, Dept Immunol, Shandong Qual Inspect Ctr Med Devices, Jinan 250100, Peoples R China.
来源：IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY
关键词：Corynebacterium parvum; Concanavalin A; Hepatitis; CD4+T cells; Nitric; oxide
摘要：Corynebacterium parvum (CP), a kind of immunomodulator, has been well documented in many diseases. Non-cell C. parvum product (NCPP) is a newly-found nano-preparation. To investigate the effect of NCPP on Con A-induced murine severe hepatitis, we pretreated mice with NCPP intraperitoneally. After 12 h, ConA (25 mu g/g body wt) was injected intravenously to provoke severe hepatitis and the degree of liver injury was evaluated by serum transaminase analysis and heptatic tissue pathology. Results have shown that levels of serum transaminase and degree of liver injury in ConA/NCPP groups had significantly declined than those in ConA/PBS groups. Notably, results of flow cytometry have demonstrated that activation of CD4+T cells in ConA/NCPP groups has been down-regulated, compared with ConA/PBS groups. Further, levels of serum and KC-related nitric oxide (NO) was displayed significantly lower in ConA/NCPP groups than those in ConA/PBS groups. The results indicate that NCPP may alleviate ConA-induced hepatitis by reducing CD4+T activation and NO production.