标题：Promotion of extracellular lignocellulolytic enzymes production by restraining the intracellular β-glucosidase in Penicillium decumbens
作者：Chen, Mei ;Qin, Yuqi ;Cao, Qing ;Liu, Guodong ;Li, Jie ;Li, Zhonghai ;Zhao, Jian ;Qu, Yinbo
作者机构：[Chen, Mei ;Qin, Yuqi ;Cao, Qing ;Liu, Guodong ;Li, Jie ;Li, Zhonghai ;Zhao, Jian ;Qu, Yinbo ] State Key Laboratory of Microbial Technology, Shandong 更多
摘要：In this study, the functions of β-glucosidases in regulation of the lignocellulolytic enzymes production in Penicillium decumbens 114-2 were investigated. The major extracellular β-glucosidase gene bgl1 and the major intracellular β-glucosidase gene bgl2 were deleted in P. decumbens 114-2 respectively. In δ. bgl2, the production of extracellular lignocellulolytic enzymes (including endoglucanases, cellobiohydrolases and xylanases) on insoluble cellulose was significantly promoted, while in δ. bgl1 there was no any difference compared with that of 114-2. The enhancement of the production of lignocellulolytic enzymes in δ. bgl2 was likely attributed to the accumulation of intracellular cellobiose. Induction experiment in δ. bgl1δ. bgl2 showed that cellobiose was an inducer of lignocellulolytic enzymes expression in P. decumbens 114-2, and the induction was unrelated to the formation, if any, of gentiobiose or sophorose from cellobiose. © 2013 Elsevier Ltd.