标题：Impaired Telomere Length and Telomerase Activity in Peripheral Blood Leukocytes and Granulosa Cells in Patients With Biochemical Primary Ovarian Insufficiency
作者：Xu, Xiaofei; Chen, Xinxia; Zhang, Xiruo; Liu, Yixun; Wang, Zhao; Wang, Peng; Du, Yanzhi; Qin, Yingying; Chen, Zi-Jiang
作者机构：[Xu, Xiaofei; Chen, Xinxia; Zhang, Xiruo; Wang, Zhao; Wang, Peng; Qin, Yingying; Chen, Zi-Jiang] Shandong Univ, Prov Hosp, Ctr Reprod Med, Jinan, Peop 更多
通讯作者：Xu, XF;Xu, XF;Xu, XF
通讯作者地址：[Xu, XF]Shandong Univ, Prov Hosp, Ctr Reprod Med, Jinan, Peoples R China;[Xu, XF]Natl Res Ctr Assisted Reprod Technol & Reprod Gen, Jinan, Peoples R C 更多
来源：OBSTETRICAL & GYNECOLOGICAL SURVEY
摘要：The underlying cause of primary ovarian insufficiency (POI) is unknown in most cases, which generally are referred to as idiopathic. Primary ovarian insufficiency is associated with biological reproductive aging and encompasses 3 stages: occult, biochemical, and overt. Telomeres-the caps of chromosomes-play an essential role in protecting chromosome structure during meiosis and meiosis ensuring accurate duplication. Telomere length is a proven biomarker for biological aging and is positively correlated with reproductive lifespan in women. It has been shown to be involved in age-related disorders such as cardiovascular diseases, neurological diseases, and cancers.; The biochemical stage of POI is recognizable using specific clinical features and offers specific treatment options. Although it is believed that changes of telomere length and telomerase activity may be associated with biochemical POI, no data on this issue have been published.; The aim of this study was to determine whether telomere length and telomerase activity are associated with biochemical POI. Participants were 120 patients with biochemical POI and 279 control women, all enrolled at a reproductive medicine center in a Chinese university. In patients with biochemical POI, relative telomere length in peripheral blood leukocytes (LTLs) and granulosa cells (GTLs) were measured using a modified quantitative polymerase chain reaction method. Relative telomerase activity (RTA) in granulosa cells was detected using a modified quantitative-telomeric repeat amplification protocol assay.; Patients with biochemical POI (n = 120) had significantly shorter LTLs (0.75 +/- 0.09 vs 1.79 +/- 0.12, P < 0.001; age-adjusted odds ratio [aOR], 0.54; 95% confidence interval [CI], 0.43-0.68) and GTLs (0.78 +/- 0.09 vs 1.90 +/- 0.23, P < 0.001; aOR, 0.54; 95% CI, 0.41-0.70) than control subjects (n = 279 for LTLs; n = 90 for GTLs). Compared with control subjects, significantly diminished RTAs were detected in granulosa cells of patients with biochemical POI (1.57 +/- 0.59 vs 4.63 +/- 0.93; aOR, 0.84; 95% CI, 0.72-0.98, P = 0.025 [n = 38 for control subjects, n = 31 for POI patients]). The cross-sectional nature of this study might have its limit in telomere length as well as telomerase activity along with the progressing decline in ovarian function.; Shortened telomere length and diminished telomerase activity were associated with biochemical POI and may be considered as indicators for progression of ovarian decline.