标题:Enzyme immobilization onto the nanomaterials: Application in enzyme stability and prodrug-activated cancer therapy
作者:Sharifi M.; Sohrabi M.J.; Hosseinali S.H.; Hasan A.; Kani P.H.; Talaei A.J.; Karim A.Y.; Nanakali N.M.Q.;等 更多
作者机构:[Sharifi, M] Department of Nanotechnology, Faculty of Advanced Sciences and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran 更多
通讯作者:Hasan, A(ahasan@qu.edu.qa)
通讯作者地址:[Hasan, A] Department of Mechanical and Industrial Engineering, College of Engineering, Qatar UniversityQatar;
来源:International Journal of Biological Macromolecules
出版年:2020
卷:143
页码:665-676
DOI:10.1016/j.ijbiomac.2019.12.064
关键词:Cancer treatment; Enzyme prodrug therapy; Enzyme stability; Immobilization; Nanomaterials
摘要:Nanoparticles (NPs) have been widely used for immobilization of wide ranges of enzymes. However, the stabilization of enzymes on NPs is a major challenge, crucial for regulating enzymatic activity and their medical applications. To overcome these challenges, it is necessary to explore how enzymes attach to nanomaterials and their properties are affected by such interactions. In this review we present an overview on the different strategies of the enzyme immobilization into the NPs and their corresponding stability against temperature and pH. The effects of surface charge, particle size, morphology, and aggregation of NPs on the stability of immobilized enzymes were summarized. The activity of immobilized enzyme into the NPs was reviewed to disclose more detail regarding the interaction of biomolecules with NPs. The combination of enzyme immobilization with prodrugs was also reviewed as a promising approach for biomedical application of enzyme in cancer therapy. Finally, the current challenges and future applications of NPs in enzyme immobilization and the utilization of immobilized enzyme toward prodrug activation in cytoplasm of cancer cells were presented. In conclusion, this review may pave the way for providing a perspective on development to the industrial and clinical translation of immobilized enzymes. © 2019
收录类别:SCOPUS
Scopus被引频次:1
资源类型:期刊论文
原文链接:https://www.scopus.com/inward/record.uri?eid=2-s2.0-85076470423&doi=10.1016%2fj.ijbiomac.2019.12.064&partnerID=40&md5=239e7e1548e8736c0b63dfbc8f8e4879
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