标题：Accelerating lipid production in freshwater alga Chlorella sorokiniana SDEC-18 by seawater and ultrasound during the stationary phase
作者：Xie, Zhen; Pei, Haiyan; Zhang, Lijie; Yang, Zhigang; Nie, Changliang; Hou, Qingjie; Yu, Ze
作者机构：[Xie, Zhen; Pei, Haiyan; Zhang, Lijie; Yang, Zhigang; Nie, Changliang; Hou, Qingjie; Yu, Ze] Shandong Univ, State Key Lab Microbial Technol, Qingdao 2 更多
通讯作者：Pei, Haiyan;Pei, Haiyan
通讯作者地址：Pei, HY (corresponding author), Shandong Prov Engn Ctr Environm Sci & Technol, Jinan 250061, Peoples R China.
关键词：Microalgae; Lipid; Seawater; Ultrasound; Two-stage cultivation
摘要：Seawater integrated with ultrasound treatment was applied to cultivate freshwater microalgae for improving both cell density and lipid content in two-stage method. In this study, Chlorella sorokiniana SDEC-18 was cultured in seawater through two stages, which comprised fast cell growth in stage I, followed by lipid stimulation in stage II to improve lipid productivity. Seawater and ultrasound, applied in stage II, improved microalgae growth greatly by removing 19% of the algal extracellular substances (AESs), and further increased the lipid accumulation from 50.53% to 64.38% in seawater. Hence, after 6 days of stage II, lipid productivity value of 28.78 mg/L/d was obtained, which was about 3.2 times more than that in the BG11 (Blue-Green Medium) control. These results prove that uniting seawater and ultrasound paves the way towards higher lipid productivity of freshwater microalgae. (C) 2020 Elsevier Ltd. All rights reserved.