标题:Conditional gene manipulation: Cre-ating a new biological era
作者:Zhang, Jian; Zhao, Jing; Jiang, Wen-jie; Shan, Xi-wei; Yang, Xiao-mei; Gao, Jian-gang
作者机构:[Zhang, J] School of Life Science, Shandong University, Jinan 250100, China;[ Zhao, J] School of Life Science, Shandong University, Jinan 250100, Chin 更多
通讯作者:Yang, XM
通讯作者地址:[Yang, XM]Shandong Univ, Sch Life Sci, Jinan 250100, Peoples R China.
来源:浙江大学学报(英文版)(B辑:生物医学和生物技术)
出版年:2012
卷:13
期:7
页码:511-524
DOI:10.1631/jzus.B1200042
关键词:Site-specific recombinase;Gene targeting;Gene trapping;Inducible systems;φC31 system
摘要:To solve the problem of embryonic lethality in conventional gene knockouts,site-specific recombinase (SSR) systems (Cre-loxP,FIp-FRT,and φC31) have been used for tissue-specific gene knockout.With the combination of an SSR system and inducible gene expression systems (tetracycline and tamoxifen),stage-specific knockout and transgenic expression can be achieved.The application of this “SSR+inducible” conditional tool to genomic manipulation can be extended in various ways.Alternatives to conditional gene targeting,such as conditional gene trapping,multipurpose conditional alleles,and conditional gene silencing,have been developed.SSR systems can also be used to construct precise disease models with point mutations and chromosomal abnormalities.With these exciting achievements,we are moving towards a new era in which the whole genome can be manipulated as we wish.
收录类别:SCOPUS;SCIE
WOS核心被引频次:11
Scopus被引频次:14
资源类型:期刊论文
原文链接:https://www.scopus.com/inward/record.uri?eid=2-s2.0-84863973946&doi=10.1631%2fjzus.B1200042&partnerID=40&md5=973737bcfc1f73750b295c59ab5c33a0
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