标题：Electrochemiluminescence of blue-luminescent graphene quantum dots and its application in ultrasensitive aptasensor for adenosine triphosphate detection
作者：Lu, Juanjuan; Yan, Mei; Ge, Lei; Ge, Shenguang; Wang, Shaowei; Yan, Jixian; Yu, Jinghua
作者机构：[Lu, Juanjuan; Yan, Mei; Ge, Lei; Wang, Shaowei; Yan, Jixian; Yu, Jinghua] Univ Jinan, Key Lab Chem Sensing & Anal, Shandong Univ, Sch Chem & Chem Eng 更多
通讯作者地址：[Yu, JH]Univ Jinan, Key Lab Chem Sensing & Anal, Shandong Univ, Sch Chem & Chem Engn, Jinan 250022, Peoples R China.
来源：BIOSENSORS & BIOELECTRONICS
关键词：Graphene quantum dots; Electrochemiluminescence; SiO2 nanospheres;; Aptamer; ATP
摘要：A simple approach based on exfoliating and disintegrating treatments for graphite oxide, followed by hydrothermal synthesis, was developed to prepare water-soluble graphene quantum dots (GQDs). The as-prepared GQDs exhibited bright blue emission under ultraviolet irradiation (similar to 365 nm), and showed an excitation-independent photoluminescence feature. More importantly, a newly anodic electrochemiluminescence (ECL) was observed from the water-soluble GQDs with H2O2 as coreactant for the first time, and the ECL induced a strong light emission at a low potential (ca. 0.4 V vs. Ag/AgCl). The ECL mechanism is investigated in detail. Employing SiO2 nanospheres as signal carrier, a novel SiO2/GQDs ECL signal amplification labels were synthesized based on which a ultrasensitive ECL aptamer sensor was proposed. Under the optimized experimental conditions, the proposed ECL aptamer sensor exhibited excellent analytical performance for adenosine triphosphate (ATP) determination, ranging from 5.0 x 10(-12) to 5.0 x 10(-9) mol L-1 with the detection limit of 1.5 x 10(-12) mol L-1. Due to the low cytotoxicity and excellent biocompatibility, GQDs are demonstrated to be an eco-friendly material as well as excellent ECL labeling agents for biosensor. (C) 2013 Elsevier B.V. All rights reserved.