标题:GLP-1/GLP-1R Signaling in Regulation of Adipocyte Differentiation and Lipogenesis
作者:Chen, Jicui; Zhao, Huichen; Ma, Xiaoli; Zhang, Yuchao; Lu, Sumei; Wang, Yangang; Zong, Chen; Qin, Dandan; Wang, Yuanmei; Yang, Yingf 更多
作者机构:[Chen, Jicui; Zhang, Yuchao; Zong, Chen; Qin, Dandan; Yang, Yingfeng; Wang, Xiangdong] Shandong Univ, Sch Med, Dept Cell Biol, Jinan 250012, Shandong, 更多
通讯作者:Wang, XD;Liu, YT
通讯作者地址:[Wang, XD]Shandong Univ, Sch Med, Dept Cell Biol, Jinan 250012, Shandong, Peoples R China;[Liu, YT]Qingdao Municipal Hosp, Dept Endocrinol, 5 Donghai 更多
来源:CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
出版年:2017
卷:42
期:3
页码:1165-1176
DOI:10.1159/000478872
关键词:Liraglutide; 3T3-L1 preadipocytes; Adipogenesis; FASN
摘要:Background/Aims: The aim of this study was to determine the direct role of liraglutide (LG) in adipogenesis and lipid metabolism. Methods: Lipid accumulation was evaluated by oil red O staining, quantitative real-time PCR (qPCR) was performed to determine glucagon-like peptide 1 receptor (GLP-1R), fatty acid synthase (FASN) and adipose triglyceride lipase (ATGL) expression in 3T3-L1 preadipocytes, differentiated adipocytes and in adipose tissues from mice. The effects of LG on 3T3-L1 adipogenesis and lipid metabolism were analyzed with qPCR, Western Blotting, oil red O staining, immunohistochemistry (IHC) and immunofluorescence (IF). All measurements were performed at least three times. Results: LG increased the expression of differentiation marker genes and lipid accumulation during preadipocyte differentiation. In differentiated adipocytes, LG decreased FASN expression, and simultaneously led to CREB phosphorylation and ERK1/2 activation which were abolished by a GLP-1R antagonist, exendin (9-39). LG induced-FASN down-regulation was partially reversed by PKA and ERK1/2 inhibitors. Consistent with above in vitro findings, LG treatment significantly reduced FASN expression in visceral adipose tissues of ob/ob mice, and reduced body weight gain. Conclusion: LG promotes preadipocytes differentiation, and inhibits FASN expression in adipocytes. LG induced down-regulation of FASN is at least partially mediated by PKA and MAPK signaling pathways. (C) 2017 The Author(s) Published by S. Karger AG, Basel
收录类别:SCOPUS;SCIE
WOS核心被引频次:2
Scopus被引频次:2
资源类型:期刊论文
原文链接:https://www.scopus.com/inward/record.uri?eid=2-s2.0-85022067328&doi=10.1159%2f000478872&partnerID=40&md5=5fb3654f1b6ff4b119ec336b753883f2
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