标题：A novel pyrazoline-based selective fluorescent probe for detecting reduced glutathione and its application in living cells and serum
作者：Wang, S.-Q.;Wu, Q.-H.;Wang, H.-Y.;Zheng, X.-X.;Shen, S.-L.;Zhang, Y.-R.;Miao, J.-Y.;Zhao, B.-X.
作者机构：[Wang, S.-Q] Institute of Organic Chemistry, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, China;[ Wu, Q.-H] Instit 更多
通讯作者地址：[Miao, JY]Shandong Univ, Sch Life Sci, Inst Dev Biol, Jinan 250100, Peoples R China.
来源：The Analyst: The Analytical Journal of the Royal Society of Chemistry: A Monthly International Publication Dealing with All Branches of Analytical Chemistry
关键词：A novel pyrazoline-based;selective fluorescent;detecting reduced
摘要：A new fluorescent probe, N-(4-(1,5-diphenyl-4,5-dihydro-1H-pyrazol-3-yl) phenyl)-2,4-dinitrobenzenesulfonamide (probe 3), was designed and synthesized as a highly sensitive and selective fluorescent probe for recognizing and detecting glutathione among biological thiols in aqueous media. Probe 3 is a nonfluorescent compound. On being mixed with biothiols under neutral aqueous conditions, the 2,4-dinitrobenzenesulfoyl moiety can be cleaved off by glutathione, and the blue emission of the pyrazoline at 464 nm is switched on, with a fluorescence enhancement of 488-fold for glutathione. Furthermore, probe 3 was highly selective for glutathione without interference from some biologically relevant analytes. The detection limit of glutathione was 4.11 × 10~(-7) M. The emission of the probe is pH independent in the physiological pH range. Moreover, the probe can be used for fluorescent imaging of cellular glutathione and can be used for detecting glutathione in calf serum.