标题：Thymosins participate in antibacterial immunity of kuruma shrimp, Marsupenaeus japonicus
作者：Feng, Xiao-Wu; Huo, Li-Jie; Yang, Ming -Chong; Wang, Jin-Xing; Shi, Xiu-Zhen
作者机构：[Feng, Xiao-Wu; Huo, Li-Jie; Yang, Ming -Chong; Wang, Jin-Xing; Shi, Xiu-Zhen] Shandong Univ, Sch Life Sci, Shandong Prov Key Lab Anim Cells & Dev Bio 更多
通讯作者地址：[Shi, XZ]Shandong Univ, Sch Life Sci, Shandong Prov Key Lab Anim Cells & Dev Biol, Jinan 250100, Shandong, Peoples R China.
来源：FISH & SHELLFISH IMMUNOLOGY
关键词：Thymosin; Antibacterial; Bacterial clearance; Survival
摘要：Thymosins beta are actin-binding proteins that play a variety of different functions in inflammatory responses, wound healing, cell migration, angiogenesis, and stem cell recruitment and differentiation. In crayfish, thymosins participate in antiviral immunology. However, the roles of thymosin during bacterial infection in shrimp remain unclear. In the present study, four thymosins were identified from kuruma shrimp, Marsupenaeus japonicus, and named as Mjthymosin2, Mjthymosin3, Mjthymosin4, and Mjthymosin5 according the number of their thymosin beta actin-binding motifs. Mjthymosin3 was selected for further study because its expression level was the highest in hemocytes. Expression analysis showed that Mjthymosin3 was upregulated in hemocytes after challenged by Vibrio anguillarum or Staphylococcus aureus. The recombinant Mjthymosin3 protein could inhibit the growth of certain bacteria in an in vitro antibacterial test. Mjthymosins could facilitate external bacterial clearance in shrimp, and were beneficial to shrimp survival post V. anguillarum or S. aureus infection. The results suggested that Mjthymosins played important roles in the antibacterial immune response of kuruma shrimp.