标题:Green route to synthesis of valuable chemical 6-hydroxynicotine from nicotine in tobacco wastes using genetically engineered Agrobacterium tumefaciens S33
作者:Yu, Wenjun; Wang, Rongshui; Li, Huili; Liang, Jiyu; Wang, Yuanyuan; Huang, Haiyan; Xie, Huijun; Wang, Shuning
作者机构:[Yu, Wenjun; Wang, Rongshui; Li, Huili; Liang, Jiyu; Wang, Shuning] Shandong Univ, Sch Life Sci, State Key Lab Microbial Technol, Jinan 250100, Shando 更多
通讯作者:Wang, Shuning
通讯作者地址:[Wang, SN]Shandong Univ, Sch Life Sci, State Key Lab Microbial Technol, Jinan 250100, Shandong, Peoples R China.
来源:BIOTECHNOLOGY FOR BIOFUELS
出版年:2017
卷:10
期:1
DOI:10.1186/s13068-017-0976-9
关键词:Nicotine; 6-Hydroxynicotine; 6-Hydroxynicotine oxidase;; Biotransformation; Functionalized pyridine; Tobacco wastes;; Agrobacterium tumefaciens
摘要:Background: Tobacco is widely planted as an important nonfood economic crop throughout the world, and large amounts of tobacco wastes are generated during the tobacco manufacturing process. Tobacco and its wastes contain high nicotine content. This issue has become a major concern for health and environments due to its toxicity and complex physiological effects. The microbial transformation of nicotine into valuable functionalized pyridine compounds is a promising way to utilize tobacco and its wastes as a potential biomass resource. Agrobacterium tumefaciens S33 is able to degrade nicotine via a novel hybrid of the pyridine and pyrrolidine pathways, in which several intermediates, such as 6-hydroxynicotine, can be used as renewable precursors to synthesize drugs and insecticides. This provides an opportunity to produce valuable chemical 6-hydroxynicotine from nicotine via biocatalysis using strain S33.; Results: To accumulate the intermediate 6-hydroxynicotine, we firstly identified the key enzyme decomposing 6-hydroxynicotine, named 6-hydroxynicotine oxidase, and then disrupted its encoding gene in A. tumefaciens S33. With the whole cells of the mutant as a biocatalyst, we tested the possibility to produce 6-hydroxynicotine from the nicotine of tobacco and its wastes and optimized the reaction conditions. At 30 degrees C and pH 7.0, nicotine could be efficiently transformed into 6-hydroxynicotine by the whole cells cultivated with glucose/ammonium/6-hydroxy-3-succinoylpyridine medium. The molar conversion and the specific catalytic rate reached approximately 98% and 1.01 g 6-hydroxynicotine h(-1) g(-1) dry cells, respectively. The product could be purified easily by dichloromethane extraction with a recovery of 76.8%, and was further confirmed by UV spectroscopy, mass spectroscopy, and NMR analysis.; Conclusions: We successfully developed a novel biocatalytic route to 6-hydroxynicotine from nicotine by blocking the nicotine catabolic pathway via gene disruption, which provides an alternative green strategy to utilize tobacco and its wastes as a biomass resource by converting nicotine into valuable hydroxylated-pyridine compounds.
收录类别:EI;SCOPUS;SCIE
Scopus被引频次:1
资源类型:期刊论文
原文链接:https://www.scopus.com/inward/record.uri?eid=2-s2.0-85037730263&doi=10.1186%2fs13068-017-0976-9&partnerID=40&md5=d89665f6a6840fdc5dd095ab52d83bfc
TOP