标题：miR-376a inhibits breast cancer cell progression by targeting neuropilin-1 NR
作者：Zhang, Lansheng; Chen, Yanwei; Wang, Hui; Zheng, Xia; Li, Caihong; Han, Zhengxiang
作者机构：[Zhang, Lansheng] Shandong Univ, Shandong Canc Hosp, Dept Radiat Oncol, Jinan, Shandong, Peoples R China.; [Zhang, Lansheng; Zheng, Xia; Li, Caihong 更多
通讯作者地址：[Han, ZX]Xuzhou Med Univ, Dept Oncol, Affiliated Hosp, 99 Huaihai West Rd, Xuzhou 221000, Jiangsu, Peoples R China.
来源：ONCOTARGETS AND THERAPY
关键词：miR-376a; NRP-1; breast cancer; Wnt/beta-catenin migration
摘要：Background: The roles and related mechanism of miR-376a in breast cancer cell progression are unclear.; Methods: Kaplan-Meier plotter analysis was used to analyze the correlation between miR-376a and the overall survival (OS) of breast cancer patients. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed to detect miR-376a level in breast cancer cells. Cell viability, transwell migration and invasion, and cell apoptosis were constructed to investigate the effects of miR-376a on breast cancer cells. Luciferase reporter and RNA immunoprecipitation (RIP) were used to explore the targeting of miR-376a on NRP-1.; Results: miR-376a expression was positively correlated with the overall survival of breast cancer patients, and significantly decreased in breast cancer cells. Functionally, miR-376a over-expression suppressed cell proliferation, migration and invasion, and promoted cells apoptosis. Additionally, miR-376a could directly target NRP-1 and exerted its effect through NRP-1.; Conclusion: miR-376a could suppress breast cancer cell progression via directly targeting NRP-1.