标题：Identification and Characterization of Diacylglycerol Acyltransferase from Oleaginous Fungus Mucor circinelloides
作者：Zhang, Luning; Zhang, Huaiyuan; Song, Yuanda
作者机构：[Zhang, Luning; Zhang, Huaiyuan; Song, Yuanda] Shandong Univ Technol, Sch Agr Engn & Food Sci, Colin Ratledge Ctr Microbial Lipids, Zibo 255049, Shand 更多
通讯作者地址：[Song, YD]Shandong Univ Technol, Sch Agr Engn & Food Sci, Colin Ratledge Ctr Microbial Lipids, Zibo 255049, Shandong, Peoples R China.
来源：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
关键词：diacylglycerol acyltransferase; Mucor circinelloides; oleaginous;; triacylglycerol
摘要：Acyl-CoA:diacylglycerol acyltransferase (DGAT) is a pivotal regulator of triacylglycerol (TAG) synthesis. The oleaginous fungus Mucor circinelloides has four putative DGATs: McDGAT1A, McDGAT1B, McDGAT2A, and McDGAT2B, classified into the DGAT1 and DGAT2 subfamilies, respectively. To identify and characterize DGATs in M. circinelloides, these four genes were expressed in Saccharomyces cerevisiae H1246 (TAG-deficient quadruple mutant), individually. TAG biosynthesis was restored only by the expression of McDGAT2B, and TAG content was significantly higher in the mutants with McDGAT2B expression than in a S. cerevisiae mutant with endogenous DGA1 expression. McDGAT2B prefers saturated fatty acids to monounsaturated fatty acids and has an obvious preference for C18:3 (omega-6) according to the results of substrate preference experiments. Furthermore, only the mRNA expression pattern of McDGAT2B correlated with TAG biosynthesis during a fermentation process. Our experiments strongly indicate that McDGAT2B is crucial for TAG accumulation, suggesting that it may be an essential target for metabolic engineering aimed at increasing lipid content of M. circinelloides.