标题:Branched-chain amino acids supplementation protects streptozotocin-induced insulin secretion and the correlated mechanism
作者:Lu, Ming;Zhang, Xiujuan;Zheng, Dongmei;Jiang, Xiuyun;Chen, Qing
作者机构:[Lu, M] Department of Endocrinology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, China;[ Zhang, X] Department of Endocrinol 更多
通讯作者:Zhang, XJ
通讯作者地址:[Zhang, XJ]Shandong Univ, Shandong Prov Hosp, Dept Endocrinol, 324 Jing 5 Rd, Jinan 250021, Shandong, Peoples R China.
来源:BioFactors
出版年:2015
卷:41
期:2
页码:127-133
DOI:10.1002/biof.1188
关键词:Streptozotocin;insulin secretion;branched-chain amino acids;JNK;pancreatic/duodenal homeobox-1;protein kinase D1
摘要:Significant evidence demonstrates that oxidative stress can impair insulin secretion and contribute to the development of type 2 diabetes. Branched-chain amino acids (BCAAs) are reported to be positively related to insulin secretion. This study aimed to determine how oxidative stress affects the function of islets and whether BCAAs can ameliorate the oxidative stress, and accompanying c-jun N-terminal kinase (JNK), protein kinase D1 (PKD1), and pancreatic/duodenal homeobox-1 (PDX-1) changes induced by streptozotocin (STZ). Plasma glucose, plasma insulin, and JNK, PKD1 and PDX-1 mRNA and protein expression were measured in rats treated with STZ and BCAAs. The glucose level in STZ-induced diabetic rats was much higher than that in control animals, and the elevated plasma glucose level in diabetic rats could be significantly inhibited by BCAAs treatment. Consistent with the change in glucose levels, the levels of insulin were also affected by BCAAs treatment. The mRNA and protein expression of JNK, PDX-1, and PKD1 were significantly altered in diabetic rats compared with the control group (P < 0.01) and treatment with a low dose of BCAA reversed these changes in those above markers significantly (P < 0.01). The present study demonstrated that STZ-induced oxidative stress could reduce serum insulin levels and alter the JNK, PDX-1, and PKD1 expression. BCAAs restored the levels of serum insulin reversed changes in JNK, PDX-1, and PKD1 expression. (C) 2014 BioFactors
收录类别:SCOPUS;SCIE
WOS核心被引频次:3
Scopus被引频次:6
资源类型:期刊论文
原文链接:https://www.scopus.com/inward/record.uri?eid=2-s2.0-84928289491&doi=10.1002%2fbiof.1188&partnerID=40&md5=b171720a39bd81f4e84151c93591c1e0
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