标题：Sepsis induced by cecal ligation and puncture
作者：Gong W.; Wen H.
作者机构：[Gong, W] Department of Hepatobiliary Surgery and Liver Transplantation, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, China; 更多
通讯作者地址：[Wen, H] Infectious Disease Institute, The Ohio State University College of MedicineUnited States;
来源：Methods in Molecular Biology
关键词：Cecal ligation and puncture; CLP; Cytokine; Macrophage; Sepsis
摘要：Despite advances in intensive care unit interventions, including the use of specific antibiotics and anti-inflammation treatment, sepsis with concomitant multiple organ failure is the most common cause of death in many acute care units. In order to understand the mechanisms of clinical sepsis and develop effective therapeutic modalities, there is a need to use effective experimental models that faithfully replicate what occurs in patients with sepsis. Several models are commonly used to study sepsis, including intravenous endotoxin challenge, injection of live organisms into the peritoneal cavity, establishing abscesses in the extremities, and the induction of experimental polymicrobial peritonitis via cecal ligation and puncture (CLP). Here, we describe the surgical procedure of CLP in mice, which has been demonstrated to closely replicate the nature and course of clinical sepsis in human subjects. © Springer Science+Business Media, LLC, part of Springer Nature 2019.