标题：Neuroprotective effects of resveratrol on damages of mouse cortical neurons induced by β-amyloid through activation of SIRT1/Akt1 pathway
作者：Jing Zhang;Xiaowen Feng;Jintao Wu;Hongyan Xu;Guibao Li;Dexiao Zhu;Qingwei Yue;Haili Liu;Yi Zhang;Dong Sun;Hui Wang;Jinhao Sun
作者机构：Department of Anatomy, Shandong University School of Medicine, Jinan, Shandong, People’s Republic of China;Department of Anatomy
通讯作者地址：[Sun, JH]Shandong Univ, Sch Med, Dept Anat, Jinan 250012, Shandong, Peoples R China.
摘要：Resveratrol (3,5,4’-tihydroxy-trans-stilbene), a polyphenolic phytoalexin found in the skin and seeds of grapes, has been reported to possess a wide range of biological and pharmacological activities including antioxidant, anti-inflammatory, and antimutagenic effects. The present study intended to explore the neuroprotective effects of resveratrol against Aβ_(25–35)-induced neurotoxicity of cultured mouse cortical neurons and the possible mechanisms involved. For this purpose, mouse cortical neurons were cultured and exposed to 30 μM Aβ_(25–35) in the absence or presence of resveratrol (5, 10, and 25 lM). In addition, the potential contribution of the SIRT1/Akt1 neuroprotective pathway in resveratrol-mediated protection against Aβ_(25–35)-induced neurotoxicity was also investigated. The results showed that resveratrol dose-dependently increased cell viability and reduced the number of apoptotic cells as measured by 3-[4,5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) assay, lactate dehydrogenase (LDH) activity assay, reactive oxygen species (ROS) activity assay, and Hoechst/PI double staining. Further study revealed that resveratrol through activation of SIRT1/Akt1 to avert apoptosis. These findings raise the possibility that resveratrol may be a potent therapeutic compound against the neurodegenerative diseases.