标题:An electrochemical immunosensor for ultrasensitive detection of carbohydrate antigen 199 based on Au@CuxOS yolk-shell nanostructures with porous shells as labels
作者:Guo, Aiping ;Li, Yueyun ;Cao, Wei ;Meng, Xianchao ;Wu, Dan ;Wei, Qin ;Du, Bin
作者机构:[Guo, Aiping ;Cao, Wei ;Meng, Xianchao ;Wu, Dan ;Wei, Qin ;Du, Bin ] Key Laboratory of Chemical Sensing and Analysis in Universities of Shandong (Univ 更多
通讯作者:Wei, Qin
来源:Biosensors and Bioelectronics
出版年:2015
卷:63
页码:39-46
DOI:10.1016/j.bios.2014.07.017
摘要:A novel and sensitive electrochemical immunosensor for ultrasensitive detection of pancreatic cancer biomarker carbohydrate antigen 199 (CA199) was proposed by using Au@CuxOS yolk-shell nanostructures with porous shells as labels for signal amplification. Au@CuxOS yolk-shell nanostructures exhibit high electrocatalytic activity toward the reduction of hydrogen peroxide (H2O2) as analytical signal. Moreover, secondary antibody (Ab2) can adsorb on the surface of Au@CuxOS with porous shells which has large surface area and could greatly increase the probability of Ab2-antigen interactions thereby leading to higher sensitivity. Reduced graphene oxide-tetraethylene pentamine (rGO-TEPA), containing abundant amine groups, was supported Au nanoparticles as a support platform to immobilize the primary antibody (Ab1). The resulting sensing interface of rGO-TEPA/AuNPs could provide a large electroconductive surface area, allowing high loadings of the biological recognition elements as well as the occurrence of electrocatalytic and electron-transfer processes. Under optimal conditions, the immunosensor exhibited a wide linear response to CA199 ranging from 0.001 to 12U/mL with a low detection limit of 0.0005U/mL. The designed immunosensor displayed good precision, high sensitivity, acceptable stability and reproducibility, and has been applied to the analysis of serum with satisfactory results. The proposed method provides a new promising platform of clinical immunoassay for other biomolecules.
© 2014 Elsevier B.V.
收录类别:EI
资源类型:期刊论文
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