标题:Triterpenoid Dihydro-CDDO-Trifluoroethyl Amide Protects against Maladaptive Cardiac Remodeling and Dysfunction in Mice: A Critical Role of Nrf2
作者:Xing, Yifan; Niu, Ting; Wang, Wenjuan; Li, Jinqing; Li, Siying; Janicki, Joseph S.; Ruiz, Stacey; Meyer, Colin J.; Wang, Xing Li; Ta 更多
作者机构:[Xing, Yifan; Zhao, Yuxia] Shandong Univ, Qilu Hosp, Dept Tradit Med, Jinan 250100, Peoples R China.; [Xing, Yifan; Niu, Ting; Wang, Wenjuan; Li, Si 更多
通讯作者:Zhao, YX
通讯作者地址:[Zhao, YX]Shandong Univ, Qilu Hosp, Dept Tradit Med, Jinan 250100, Peoples R China.
来源:PLOS ONE
出版年:2012
卷:7
期:9
DOI:10.1371/journal.pone.0044899
摘要:Background and Aims: Nuclear factor E2-related factor 2 (Nrf2) appears to be an attractive therapeutic target for the treatment of cardiac disease. We investigated whether a synthetic triterpenoid derivative of dihydro-CDDO-trifluoroethylamide (dh404), a novel Nrf2 activator, protects against pathological cardiac responses to hemodynamic stress in mice.; Methods: Cardiac maladaptive remodeling and dysfunction were established by transverse aortic constriction (TAC) in mice. Hypertrophic growth of rat neonatal cardiomyocytes was induced by angiotensin II (Ang II). Cell death of rat neonatal cardiomyocytes was induced with hydrogen peroxide (H2O2). Cellular proliferation of rat neonatal cardiac fibroblasts was induced by Ang II, norepinephrine (NE) and phenylephrine (PE). Protein expression was assessed by immunochemical staining and Western blots. Gene expression was determined by real time reverse transcription-polymerase chain reaction (Q-PCR).; Results: TAC suppressed myocardial Nrf2 expression, increased myocardial 4-hydroxy-2-nonenal and 8-hydroxydeoxyguanosine levels, and induced cardiac hypertrophy, fibrosis and apoptosis, and overt heart failure and death in mice. Administration of dh404 inhibited the pathological cardiac remodeling and dysfunction, and reduced the mortality. Moreover, dhd404 elevated myocardial levels of Nrf2 and Nrf2 nuclear translocation with a dramatic suppression of the oxidative stress in the heart. Dh404 inhibited hypertrophic growth and death in primary culture of rat neonatal cardiomyocytes and suppressed proliferation in primary culture of rat neonatal cardiac fibroblasts. However, these effects of dh404 were blunted by knocking down of Nrf2.; Conclusion: These findings demonstrate that dh404 prevents pathological cardiac remodeling and dysfunction by activating Nrf2, indicating a therapeutic potential of dh404 for cardiac disease.
收录类别:SCOPUS;SCIE
WOS核心被引频次:24
Scopus被引频次:28
资源类型:期刊论文
原文链接:https://www.scopus.com/inward/record.uri?eid=2-s2.0-84866456998&doi=10.1371%2fjournal.pone.0044899&partnerID=40&md5=12356a33240a76b8cf13d4ff7a7e313b
TOP