标题：Facile Enzymatic Synthesis of Phosphorylated Ketopentoses
作者：Wen, Liuqing; Huang, Kenneth; Liu, Yunpeng; Wang, Peng George
作者机构：[Wen, Liuqing; Huang, Kenneth; Liu, Yunpeng; Wang, Peng George] Georgia State Univ, Dept Chem, Atlanta, GA 30303 USA.; [Liu, Yunpeng; Wang, Peng Geo 更多
通讯作者地址：[Liu, YP; Wang, PG]Georgia State Univ, Dept Chem, Atlanta, GA 30303 USA;[Liu, YP; Wang, PG]Shandong Univ, Natl Glycoengn Res Ctr, Jinan 250100, People 更多
关键词：biocatalysis; enzymatic synthesis; phosphorylated ketopentoses; one-pot; reactions; silver nitrate precipitation
摘要：An efficient and convenient platform for the facile synthesis of phosphorylated ketoses is described. All. eight phosphorylated ketopentoses were produced using this platform starting from two common and inexpensive aldoses (D-xylose and L-arabinose) in more than 84% isolated yield (gram scale). In this method, reversible conversions (isomerization or epimerization) were accurately controlled toward the formation of desired ketose phosphates by targeted phosphorylation reactions catalyzed by substrate-specific kinases. The byproducts were selectively removed by silver nitrate precipitation avoiding the tedious and time-consuming separation of sugar phosphate from adenosine phosphates (ATP and ADP). Moreover, the described strategy can be expanded for the synthesis of other sugar phosphates.