标题:Upregulation of miR-33b promotes endometriosis via inhibition of Wnt/-catenin signaling and ZEB1 expression
作者:Zhang, Haiyan; Li, Guang; Sheng, Xiugui; Zhang, Shiqian
作者机构:[Zhang, Haiyan; Zhang, Shiqian] Shandong Univ, Affiliated Qilu Hosp, Dept Gynecol, 107 Culture West Rd, Jinan 250002, Shandong, Peoples R China.; [Z 更多
通讯作者:Zhang, SQ
通讯作者地址:[Zhang, SQ]Shandong Univ, Affiliated Qilu Hosp, Dept Gynecol, 107 Culture West Rd, Jinan 250002, Shandong, Peoples R China.
来源:MOLECULAR MEDICINE REPORTS
出版年:2019
卷:19
期:3
页码:2144-2152
DOI:10.3892/mmr.2019.9870
关键词:microRNA-33b; endometriosis; Wnt; -catenin; zinc finger E-box-binding; homeobox 1
摘要:The present study aimed to investigate the role and mechanisms of microRNA (miR)-33b in endometriosis (Ems). Reverse transcription-quantitative polymerase chain reaction (RT-qPCR), MTT assays, flow cytometry, caspase-3/9 activity assays and western blotting were performed in the present study. Initially, miR-33b expression in an Ems rat model was investigated by RT-qPCR and was demonstrated to be upregulated in Ems tissue samples of rats compared with the control group. In addition, miR-33b upregulation inhibited cell growth and enhanced apoptosis in an Ems model (primary cell cultures) compared with the control group. In addition, miR-33b up-regulation reduced Wnt/-catenin signaling pathway and suppressed zinc finger E-box-binding homeobox 1 (ZEB1) protein expression in the in vitro Ems model (primary cell cultures) compared with the control group. Furthermore, small interfering-ZEB1 ameliorated the effects of miR-33b downregulation on Ems cell growth in the in vitro Ems model. Additionally, a Wnt agonist reduced the effects of miR-33b upregulation on Ems cell growth in the in vitro Ems model. In conclusion, the present study demonstrated that upregulation of miR-33b may promote Ems through Wnt/-catenin by ZEB1 expression.
收录类别:SCOPUS;SCIE
资源类型:期刊论文
原文链接:https://www.scopus.com/inward/record.uri?eid=2-s2.0-85061147938&doi=10.3892%2fmmr.2019.9870&partnerID=40&md5=5efb430b451ff3ec17bcf6bc82573fa8
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