标题:Modified acellular porcine corneal matrix in deep lamellar transplantation of rabbit cornea
作者:Wang Q.; Zhou H.; Sun Y.; Cao C.; Pang K.
作者机构:[Wang, Q] School of Chemistry and Chemical Engineering, Shandong University, Jinan, China;[ Zhou, H] School of Chemistry and Chemical Engineering, Sha 更多
通讯作者:Wang, Q(allenkp@163.com)
通讯作者地址:[Wang, Q] School of Chemistry and Chemical Engineering, Shandong UniversityChina;
来源:Journal of Biomaterials Applications
出版年:2020
DOI:10.1177/0885328219898372
关键词:1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide; Corneal tissue engineering; crosslinking; decellularization; enhanced biocompatibility
摘要:This study presents to develop a modified acellular porcine corneal matrix (MAPCM) to maintain high transparency, stability and biocompatibility as a rabbit deep cornea replacement using 1-ethyl-3–(3-dimethylaminopropyl)-carbodiimide crosslinking and a mild decellularization technique. Scaffolds are translucent and remain higher amount of glycosaminoglycans after decellularization than acellular porcine corneal matrix (APCM). Enzymatic degradation kinetics and mechanical properties of scaffolds are regulated by 1-ethyl-3–(3-dimethylaminopropyl)-carbodiimide -crosslinking density. The porous structure and ultrastructure of collagenous lamellae are maintained, and the pore size of MAPCM crosslinked with 0.5% (w/v) 1-ethyl-3–(3-dimethylaminopropyl)-carbodiimide is 13.26 ± 1.65 µm, similar to that of normal porcine cornea. The transmittance of MAPCM gets 79.1 ± 0.45 to 92.7 ± 1.4% in the visible light range. Results from a CCK-8 assay indicate that MAPCM gets higher cell proliferation rate of rabbit corneal stroma cells than APCM. Since collagen fibres structural integrity and regularity of MAPCM are retained after crosslinking, the opacity and stability of MAPCM are better than those of APCM within 4 weeks of animal implantation. In addition, there is no indication of an immune response or neovascularization in or around the transplanted disc. These results reveal that MAPCM may be a more suitable scaffold for corneal substitute construction. © The Author(s) 2020.
收录类别:SCOPUS
资源类型:期刊论文
原文链接:https://www.scopus.com/inward/record.uri?eid=2-s2.0-85077565953&doi=10.1177%2f0885328219898372&partnerID=40&md5=7844fac3d8cd38a2b66142489b72d74c
TOP