标题：Influence of neonatal gender on cord blood CD34(+) cell amplification and gene expression
作者：Zhou, Liping; Che, Zhe; Zhang, Xiaowei; Zhou, Panpan; Li, Xue; Xu, Xuejing; Shi, Qing; Li, Dong; Ju, Xiuli
作者机构：[Zhou, Liping; Zhang, Xiaowei] Sixth Peoples Hosp Jinan, Dept Pediat, Jinan 250200, Shandong, Peoples R China.; [Zhou, Liping; Zhou, Panpan; Li, Xue 更多
通讯作者：Li, D;Ju, XL
通讯作者地址：[Li, D; Ju, XL]Shandong Univ, Qilu Hosp, Cryomed Lab, 17 Wen Hua Xi Rd, Jinan 250012, Shandong, Peoples R China.
来源：EXPERIMENTAL AND THERAPEUTIC MEDICINE
关键词：gender; cord blood; hematopoietic stem cells; amplification; gene; expression
摘要：The present study attempted to evaluate whether neonatal gender affects the hematopoietic potential of cord blood (CB) transplants and, if so, to determine the underlying molecular mechanisms. CD34(+) cells from CB were isolated and divided into male and female groups. CD34(+)CD38(-) cell populations were then compared using fluorescence-assisted cell sorting (FACS) and a colony formation assay was performed. Next, a Genechip microarray analysis was used to identify differentially expressed genes (DEGs). Finally, the Genechip results were validated by FACS analysis. It was revealed that the male group had higher amplification efficiency. Gene ontology analysis indicated differences in the biological function of the DEGs between the two groups. Kyoto Encyclopedia of Genes and Genomes analysis suggested that the hematopoietic cell lineage signaling pathway was upregulated in the male group along with high expression levels of genes including interleukin (IL) 6 signal transducer (glycoprotein 130), IL-7 and IL-7 receptor. It was speculated that this may be partially due to numerous upregulated DEGs being involved in chromosomal segregation and hematopoietic cell lineage signaling pathways in CD34(+) cells from the male group.