标题：A photoelectrochemical biosensor for fibroblast-like synoviocyte cell using visible light-activated NCQDs sensitized-ZnO/CH3NH3PbI3heterojunction
作者：Pang, Xuehui ;Zhang, Yong ;Pan, Jihong ;Zhao, Yanxia ;Chen, Yao ;Ren, Xiang ;Ma, Hongmin ;Wei, Qin ;Du, Bin
作者机构：[Pang, Xuehui ;Zhang, Yong ;Ren, Xiang ;Ma, Hongmin ;Wei, Qin ;Du, Bin ] Key Laboratory of Chemical Sensing and Analysis in Universities of Shandong, 更多
来源：Biosensors and Bioelectronics
摘要：Based on ZnO nanorods (NRs)/CH3NH3PbI3/nitrogen-doped carbon quantum dots (NCQDs) nanocomposites, the highly sensitive detection of fibroblast-like synoviocyte (FLS) cell was realized by a photoelectrochemical (PEC) biosensor. ZnO/CH3NH3PbI3/NCQDs nanocomposites were exploited as the photo-to-electron generator to produce the signal. CH3NH3PbI3was spin-coated on ZnO surface after ZnO NRs grew on ITO electrode then by dropping on the modified electrode, NCQDs were diffused and adhered to the surface of ZnO and CH3NH3PbI3. In the presence of EDC/NHS, the combination of CH3NH3PbI3and NCQDs was achieved by the carboxyl groups (-COOH) and amino groups (-NH2) in the preparation process. Furthermore, the capture probe of FLS cell, CD95 antibody, can be anchored by -COOH and -NH2groups through EDC/NHS. The specific recognition between the antibody capture probes and cell targets gained high-sensitive detection for FLS cell for the first time. The developed biosensor showed a wide linear range from 1.0×104cell/mL to 10cell/mL and a low detection limit of 2cell/mL. This kind of biosensor would provide a novel detection strategy for FLS cell.
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