标题：A competitive-type photoelectrochemical immunosensor for aflatoxin B1 detection based on flower-like WO3as matrix and Ag2S-enhanced BiVO4for signal amplification
作者：Feng, Jinhui ;Li, Yueyun ;Gao, Zengqiang ;Lv, Hui ;Zhang, Xiaobo ;Dong, Yunhui ;Wang, Ping ;Fan, Dawei ;Wei, Qin
作者机构：[Feng, Jinhui ;Li, Yueyun ;Gao, Zengqiang ;Lv, Hui ;Zhang, Xiaobo ;Dong, Yunhui ;Wang, Ping ] School of Chemistry and Chemical Engineering, Shandong U 更多
来源：Sensors and Actuators, B: Chemical
摘要：A competitive photoelectrochemical (PEC) immunosensor was successfully utilized for quantitative detection of aflatoxin B1 (AFB1). The flower-like tungsten oxide (WO3) hierarchical architectures with large specific surface areas and porous nanostructure was prepared as a matrix material for immobilizing of BSA-AFB1. The Ag ions-modified bismuth vanadate nano-polyhedron (BiVO4@Ag+) was utilized as labels for immobilization anti-AFB1 through affinity specific binding. The Ag2S was prepared in-situ growth by immediately deposition the S2−onto the BiVO4@Ag+. The high photocurrent intensity was generated by the obtained BiVO4@Ag2S under visible-light irradiation. Moreover, excellent sensitivity was achieved by using the WO3as the matrix and BiVO4@Ag2S as labels to enhance photocurrent response and improve the photocurrent conversion efficiency. Under optimal conditions, the competitive PEC immunosensor showed wide linear range from 1.0 pg mL−1to 120 ng mL−1with limit detection of 0.28 pg mL−1for AFB1. The biosensor was performed with good reproducibility, acceptable stability, and high specificity, which indicated potential application in the detection of other toxic small molecules.
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