标题:A new rhodamine B-based lysosomal pH fluorescent indicator
作者:Lv, Hong-Shui; Huang, Shu-Ya; Zhao, Bao-Xiang; Miao, Jun-Ying
作者机构:[Lv, Hong-Shui; Zhao, Bao-Xiang] Shandong Univ, Inst Organ Chem, Sch Chem & Chem Engn, Jinan 250100, Peoples R China.; [Huang, Shu-Ya; Miao, Jun-Yin 更多
通讯作者:Zhao, BX
通讯作者地址:[Zhao, BX]Shandong Univ, Inst Organ Chem, Sch Chem & Chem Engn, Jinan 250100, Peoples R China.
来源:ANALYTICA CHIMICA ACTA
出版年:2013
卷:788
页码:177-182
DOI:10.1016/j.aca.2013.06.038
关键词:Rhodamine B; Fluorescent probe; Intracellular pH; Living cells;; Lysosomes
摘要:We designed and synthesized a new pH fluorescent probe, RCE, based on structural changes of rhodamine dye at different pH values. The probe exhibits high selectivity, high sensitivity and quick response to acidic pH, as well as low cytotoxicity, excellent photostability, reversibility and cell membrane permeability. Fluorescence intensity at 584 nm was increased more than 150-fold within pH range 7.51-3.53. This probe has pK(a) value 4.71, which is valuable for studying acidic organelles. Because of its long absorption and emission wavelengths, RCE can avoid associated cell damage. The probe can selectively stain lysosomes and monitor lysosomal pH changes in living cells. (C) 2013 Elsevier B.V. All rights reserved.
收录类别:EI;SCOPUS;SCIE
WOS核心被引频次:69
Scopus被引频次:71
资源类型:期刊论文
原文链接:https://www.scopus.com/inward/record.uri?eid=2-s2.0-84880042902&doi=10.1016%2fj.aca.2013.06.038&partnerID=40&md5=da14ab4b51d07ccfab4e334f6bdfaadb
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