标题：Isolation and characterization of an antioxidant exopolysaccharide produced by Bacillus sp. S-1 from Sichuan Pickles
作者：Hu, Xinyuan; Pan, Xin; Wang, Peng George; Chen, Min
作者机构：[Hu, Xinyuan; Pan, Xin; Wang, Peng George; Chen, Min] Shandong Univ, Sch Life Sci, State Key Lab Microbial Technol, Jinan, Shandong, Peoples R China.; 更多
通讯作者：Chen, Min;Chen, M;Chen, M
通讯作者地址：[Chen, M]Shandong Univ, Sch Life Sci, State Key Lab Microbial Technol, Jinan, Shandong, Peoples R China;[Chen, M]Shandong Univ, Natl Glycoengn Res Ctr 更多
关键词：Bacillus sp.; Exopolysaccharides; Free radicals; Antioxidant activity
摘要：It is urgent to explore food-grade EPSs from edible microorganism. In this study, a new type of exopolysaccharide (EPS) secreted by Bacillus sp. S-1 from Sichuan Pickles was isolated and fractioned on anion-exchange and gel-filtration chromatography. As indicated by monosaccharides components analysis, the major component BS-2 mainly consisted of galactose, glucose, and mannose with average molecular weight (Mw) of 1.765x10(4) Da. Moreover, the characterization of EPS was investigated by FT-IR and NMR Spectroscopy. In vitro antioxidant activity assay, it proved that the EPSs possessed potent quenching capacities on superoxide, hydroxyl, and DPPH radicals in a dose-dependent way. Furthermore, the major component BS-2 showed potent protection against oxidative injury by inhibiting H2O2-induced apoptosis in RAW264.7 cells. As suggested by the results, BS-2 might serve as a promising antioxidant agent to be applied in nutraceutical and pharmaceutical industries.