标题:PREPARATIVE ISOLATION AND PURIFICATION OF FLAVONOIDS FROM CUSCUTA CHINENSIS LAM. BY HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY
作者:Pan, Shaobin; Wang, Xiao; Duan, Wenjuan; Yu, Zongyuan; Zhang, Lin; Liu, Wei
作者机构:[Pan, Shaobin; Wang, Xiao; Duan, Wenjuan; Zhang, Lin; Liu, Wei] Shandong Acad Sci, Shandong Anal & Test Ctr, Jinan 250014, Shandong, Peoples R China.; 更多
通讯作者:Liu, W
通讯作者地址:[Liu, W]Shandong Acad Sci, Shandong Anal & Test Ctr, 19 Keyuan St, Jinan 250014, Shandong, Peoples R China.
来源:JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
出版年:2014
卷:37
期:15
页码:2162-2171
DOI:10.1080/10826076.2013.825869
关键词:identification; Cuscuta chinensis Lam.; NMR; flavonoid; separation;; HSCCC
摘要:Five flavonoids, quercetin-3-O-beta-D-apiofuranosyl-(1 -> 2)-beta-D-galactoside, hyperoside, kaempferol-3-O-beta-D-glucoside, kaempferol, quercetin, and chlorogenic acid, have been isolated and purified for the first time from the seeds of Cuscuta chinensis Lam. By high-speed countercurrent chromatography (HSCCC). Ethyl acetate extract (700mg) was separated by HSCCC using solvent system [ethyl acetate-ethanol-water-acetic acid (4:1:5:0.2, v/v)], yielding 34mg of quercetin-3-O-beta-D-apiofuranosyl-(1 -> 2)-beta-D-galactoside, 27.5mg of chlorogenic acid, 86mg of hyperoside, and 12mg of kaempferol-3-O-beta-D-glucoside. Then we collected tail blowing fluid and evaporated to dryness, obtaining 196mg of extract. Afterwards 196mg of extract used solvent system [chloroform-methanol-water (4:3:2, v/v)] by HSCCC, gaining 53mg of kaempferol and 18mg of quercetin. The purities of six components reach over 95%. The chemical structures of these compounds were identified by electrospray ionization mass spectrometry (ESI-MS), H-1-NMR, and C-13-NMR.
收录类别:EI;SCIE
WOS核心被引频次:4
资源类型:期刊论文
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