标题：Bioreceptor multi-walled carbon nanotubes@Fe3O4@SiO2-surface molecular imprinted polymer in an ultrasensitive chemiluminescent biosensor for bovine hemoglobin
作者：Duan, Huimin ;Wang, Xiaojiao ;Wang, Yanhui ;Li, Jianbo ;Luo, Chuannan
作者机构：[Duan, Huimin ;Wang, Xiaojiao ;Wang, Yanhui ;Li, Jianbo ;Luo, Chuannan ] Key Laboratory of Chemical Sensing and Analysis in Universities of Shandong ( 更多
摘要：An ultrasensitive chemiluminescent biosensor with a high selectivity, based on a bioreceptor surface molecular imprinted polymer (SMIP) that used core-shell Fe3O4@SiO2-multi-walled carbon nanotube nanostructures (Fe3O4@SiO2/MWCNTs) as the backbone material, for bovine hemoglobin (BHb) determination was proposed. The Fe3O4@SiO2/MWCNTs were synthesized with a new method, and then were characterized using SEM, FTIR and XRD techniques. The adsorption ability of Fe3O4@SiO2/MWCNTs-SMIP was evaluated to be 91 mg g-1following the Langmuir isotherm equation and it demonstrated an excellent recognition and adsorption ability for the imprinted sites located on the surface or near the surface of the Fe3O4@SiO2/MWCNTs. Under optimum conditions of CL, the detection range of BHb was from 5.0 × 10-10to 7.0 × 10-7mg mL-1with a detection limit of 1.5 × 10-10mg mL-1(3δ). The proposed biosensor was successfully applied in the determination of BHb in real samples with high selectivity and sensitivity, and the recoveries were excellent and varied from 92% to 106%. Finally, the possible CL mechanism of the BHb in amplifying the CL signal of the luminol-NaOH-H2O2system was discussed.
© The Royal Society of Chemistry 2015.