标题：Artesunate possesses anti-leukemia properties that can be enhanced by arsenic trioxide
作者：Li, Ying; Feng, Lili; Li, Ying; Jiang, Wen; Shan, Ningning; Wang, Xin
作者机构：[Li, Ying; Feng, Lili; Li, Ying; Shan, Ningning; Wang, Xin] Shandong Univ, Prov Hosp, Dept Hematol, Jinan 250021, Shandong, Peoples R China.; [Li, Y 更多
通讯作者地址：[Wang, X]Shandong Univ, Prov Hosp, 324 Jingwu Weiqi Rd, Jinan 250021, Shandong, Peoples R China.
来源：LEUKEMIA & LYMPHOMA
关键词：Artesunate; anti-leukemia; arsenic trioxide
摘要：Artesunate (ART), an effective and safe anti-malaria drug, also exhibits anticancer activity. We studied the effects of ART on proliferation and apoptosis of human K562 and U937 leukemia cell lines. MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay demonstrated that ART inhibits cell growth in a dose- and time-dependent manner. Based on the findings obtained from light, fluorescence and transmission electron microscopy and propidium iodide staining, the effect of ART was shown to be mediated through apoptosis. In parallel, ART treatment increased Fas expression, while it decreased the c-Fos level in K562 cells. Furthermore, co-treatment with arsenic trioxide (ATO) significantly facilitated ART-induced K562 cell apoptosis. These findings demonstrated that ART had an antitumor activity against K562 and U937 leukemia cells, largely due to inhibition of proliferation and induction of apoptosis via the intrinsic pathway; and this tumoricidal function could be enhanced by ATO.