标题：Eriodictyol protects against H 2O 2-induced neuron-like PC12 cell death through activation of Nrf2/ARE signaling pathway
作者机构：[Lou, H] Department of Pharmacology, School of Medicine, Shandong University, 44 Wenhua Xi Road, Jinan, Shandong Province, China;[ Jing, X] Department 更多
通讯作者地址：[Zhang, XM]Shandong Univ, Dept Pharmacol, Sch Med, 44 Wenhua Xi Rd, Jinan 250012, Shandong, Peoples R China.
来源：Neurochemistry International: The International Journal for the Rapid Publication of Critical Reviews, Preliminary and Original Research Communications in Neurochemistry
摘要：Eriodictyol, a flavonoid isolated from the Chinese herb Dracocephalum rupestre has long been established as an antioxidant. The present study was designed to explore the protective effects of eriodictyol against hydrogen peroxide (H 2O 2)-induced neurotoxicity with cultured rat pheochromocytoma cells (PC12 cells) and the possible mechanisms involved. For this purpose, differentiated PC12 cells were cultured and exposed to 200 μM H 2O 2 in the absence or presence of eriodictyol (20, 40 and 80 μM). In addition, the potential contribution of the Nrf2/ARE neuroprotective pathway in eriodictyol-mediated protection against H 2O 2-induced neurotoxicity was also investigated. The results showed that H 2O 2-induced cell death can be inhibited in the presence of eriodictyol as measured by assays for MTT and apoptosis. Further study revealed that eriodictyol induced the nuclear translocation of Nrf2, enhanced the expression of heme oxygenase (HO-1) and γ-glutamylcysteine synthetase (γ-GCS), and increased the levels of intracellular glutathione. Treatment of PC12 cells with Nrf2 small interference RNA abolished eriodictyol-induced HO-1 and γ-GCS expression and its protective effects. In conclusion, these results suggest that eriodictyol upregulates HO-1 and γ-GCS expression through the activation of Nrf2/ARE pathway and protects PC12 cells against H 2O 2-induced oxidative stress.